粒细胞集落刺激因子促进大鼠移植肝的再生作用
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陈国栋, 刘玉兰, 尤 鹏, 陈 宁
肝移植;粒细胞集落刺激因子;肝再生;免疫组化;增殖细胞和抗原,陈国栋,刘玉兰,尤鹏,陈宁,陈国栋,通讯作者,Granulocytecolonystimula
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陈国栋, 刘玉兰, 尤鹏, 陈宁, 北京大学人民医院消化科, 北京大学器官移植中心 北京市 100044
陈国栋, 2003年北京大学医学部硕士, 北京大学人民医院消化科住院医师, 主要从事肝移植免疫的研究.
通讯作者: 陈国栋, 100044, 北京市西直门南大街11号, 北京大学人民医院消化科. absnmj@hotmail.com
收稿日期: 2006-04-05 接受日期: 2006-04-24
Granulocyte colony stimulating factor accelerates regeneration and attenuates injury of partial liver allograft in rats
Guo-Dong Chen, Yu-Lan Liu, Peng You, Ning Chen
Guo-Dong Chen Yu-Lan Liu, Peng You, Ning Chen, Department of Gastroenterology, People’s Hospital; Center for Organ Transplantation, Peking University, Beijing 100044, China
Correspondence to: Guo-Dong Chen, Department of Gastroenterology, People’s Hospital, Peking University, 11 Xizhimen South Street, Beijing 100044, China. absnmj@hotmail.com
Received: 2006-04-05 Accepted: 2006-04-24
Abstract
AIM: To investigate the effect of granulocyte colony-stimulating factor (G-CSF) on the rege-neration of partial liver allograft in rats.
METHODS: Rat models with 50% partial liver transplantation (PLTx) were established, followed by administration of either saline or G-CSF for 5 consecutive days. Livers and serum samples were harvested 1 3, 5, 7 and 14 d after PLTx. GRWR (graft-recipient weight ratio) and serum biochemical parameters were calculated or measured, and the expression of PCNA (proliferating cell nuclear antigen) was detected by immunohistochemistry (SABC).
RESULTS: As compared with that in control group, the survival rate of liver allograft was significantly higher (90% vs 60%, χ2 = 5.03, P < 0.05) in G-CSF groups. Three days after PLTx, liver regeneration reached the peak in both groups. In comparison with those in the controls, GRWR was increased (P < 0.05), and the level of aspartate transaminase (AST) and alanine aminotransferase (ALT) were lower (3 d: t = 17.61, P < 0.05; t = 20.16, P < 0.05; 5 d: t = 15.64, P < 0.05; t = 23.08, P < 0 ......
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