【摘要】 目的 建立体外有效扩增脐带血NKT细胞的方法及其表型的研究。方法 分别采用单加IL-2，及同时加IL-2和α-半乳糖神经酰胺(α-Galcer)的方法，从人脐带血单个核细胞(UCB-MNCs)中扩增NKT细胞。用流式细胞检测技术测定NKT细胞(TCR Vα24+ TCR Vβ11+)的比例及其他表型特征。结果 UCB-MNCs在第7天时，其TCR Vαβ+ NKT细胞的扩增量占淋巴细胞的(24.48±4.19)%，是原来的(8.74±4.37)×102倍 ( P<0.01)，且大多不表达NK1.1(CD161)；TCR Vβ11+较TCR Vα24+高表达。结论 α-Galcer对脐血NKT细胞有特异地扩增功能。以表型划分，脐血中的NKT细胞多为NK1.1-，一种新的NKT细胞亚型。

    【关键词】 脐带血； NKT细胞；α-Galcer；细胞表型

    The proliferation of natural killer T cells in umbilical cord blood and the different phenotype between them

    LIU Yan, FAN Hua-hua, RUAN Ming,et al.Shanghai Blood Center, Shanghai 200051,China.

    【Abstract】 Objective The first objective of this study was to establish an effective method to proliferate natural killer T (NKT) cells in umbilical cord blood (UCB). The second one was to study the phenotype.Methods Mononuclear cells (MNCs) from UCB were cultured in the presence of IL-2 (100U/ml), with or without alpha-galcer. TCRValpha24Vbeta11 double positive NKT cells and its other phenotypes were determined by flow cytometry. Results After 7 days expansion, TCRVαβ+ NKT cells from UCB-MNCs increased(8.74±4.37)×102 times, but most of them did not express NK1.1 and its TCR Vβ11+ was higher than TCR Vα24+. Conclusion Human TCRValpha24Vbeta11 double positive NKT cells can expand following the administration of alpha-galcer. Most of the UCB-NKT was NK1.1-, a new subset of NKT cells. ......