人肝癌细胞__(期刊论文)

脂质体介导人纤溶酶原k13基因诱导人肝癌细胞凋亡

http://www.100md.com 《广东药学院学报》 2005年第3期

人肝癌细胞,,人纤溶酶原k13基因；阳离子脂质体；人肝癌细胞；细胞凋亡,1材料与方法,2结果,3讨论,参考文献：

     摘要:目的观察脂质体介导人纤溶酶原k13基因诱导体外培养人肝癌细胞凋亡的效应。方法构建人纤溶酶原k13基因真核表达载体pcDNAk13并以阳离子脂质体介导pcDNAk13转染体外培养人肝癌细胞，经Heochst 33258染色后，在荧光显微镜下观察细胞核的形态。结果转染pcDNAk13组可见致密浓染的凋亡细胞核。结论脂质体介导人纤溶酶原k13基因能诱导体外培养人肝癌细胞凋亡。

    关键词:人纤溶酶原k13基因；阳离子脂质体；人肝癌细胞；细胞凋亡

    Human Plasminogen k13 gene induces apoptosis in human hepatocellular carcinoma cells by polycationic liposomemediated transfection

    LU Xingyan，ZHANG Tianyuan，LUO Liqiong

    (1. Department of Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou,510240,China；2. Key Laboratory of Gene Engineering of State Ministry of Education, Sun Yatsen University, Guangzhou 510275,China)

    Abstract: Objective To investigate the effect on apoptosis of cultured human hepatocellular carcinoma (HCC) cells induced by the human plasminogen kringle 13 (k13) gene by polycationic liposomemediated transfection. Methods recombinant eukaryotic expression vectors pcDNAk13 encoding human plasminogen k13 gene were constructed and transferred to cultured HCC cells by polycationic liposomemediated gene transfer method. After stained with Hoechst 33258 staining solution, and the stained nuclei were observed under a fluorescence microscope. Results condensed bright apoptotic nuclei were readily observed among cells with transfected pcDNAk13. Conclusion human plasminogen k13 gene induces apoptosis in cultured HCC cells by polycationic liposomemediated transfection. ......

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