摘要：目的：评估血管紧张素转换酶(ACE)基因、血管紧张素原基因(AGT)及内皮型一氧化氮合酶基因(eNOS)多态性与冠心病的关系,并分析ACE 、AGT和eNOS基因多态性在冠心病的发生和发展中是否存在协同作用。方法：选择冠心病患者133人及对照者154人，用基因芯片技术检测ACE、AGT和eNOS基因多态性，并对比其基因型及等位基因频率。结果：冠心病组ACE DD ，AGT TT和eNOS TT基因型频率与对照组相比有显著性差异，分别为OR=2.17，P<0.01，OR=2.63，P<0.001， OR=8.50，P<0.05。ACE、AGT和 eNOS基因多态性与冠心病明显相关。同时携带ACE DD 和AGT TT基因型或AGT TT和eNOS TT基因型者与冠心病明显相关(OR=3.47，P<0.01， OR=1.05，P<0.01)。结论：ACE、AGT和 eNOS基因多态性可能是中国人冠心病的危险因素。基因芯片技术为研究多种易感基因与冠心病的相关性提供了一项高效、敏感的方法。

    关键词： 冠状动脉疾病； 血管紧张素转换酶； 血管紧张素原； 内皮型一氧化氮合酶基因； 基因芯片

    The Research of AC Angiotensinogen and Endothelial Nitric Oxide Synthase gene Polymorphisms in Predisposition to CHD with Gene Chip Technology

    LIANG Xi, et al

    (The First People’s Hospital of Foshan City, Guangdong Foshan 528000, China)

    Abstract： Objective： To evaluate the involvement of Angiotensin-converting enzyme(ACE)，Angiotensinogen (AGT) and endothelial nitric oxide synthase (eNOS) gene polymorphisms in predisposition to coronary heart disease(CHD) as well as their possible synergetic effect in the development of CHD. Method： The study included 287 subjects (133 with CHD and 154 controls). Gene chip technology was performed. The allele frequency and genotype distribution were compared between groups. Result： Significantly higher frequencies of the DD genotype of ACE gene, TT genotypes of AGT and eNOS gene were observed in CHD group. The ACE,AGT and eNOS gene polymorphisms were associated with CHD(OR=2.17 ......