【摘要】 目的 研究丙戊酸(VPA)、蛋氨酸(Methionine，Met)对大鼠皮质神经元谷氨酸脱羧酶(GAD)表达的影响，探讨VPA对精神分裂症的治疗作用。方法 采用原代培养技术分组培养大鼠皮质神经元，收集细胞、制备GAD粗酶提取液，用比色法测定粗酶提取液GAD活力；分析空白组(无血清培养液)、Met组(无血清培养液含终浓度为2mM的Met培养3天后换以无血清培养液)、VPA组(无血清培养液含终浓度为2mM的Met培养3天后换以终浓度为0.5mM的VPA 无血清培养液)的GAD活性。结果 Met组GAD平均酶活力明显低于空白组，差异有统计学意义，而VPA组的GAD平均酶活力接近于空白组，差异无统计学意义。结论 蛋氨酸抑制大鼠皮质神经元表达GAD，降低GABA的合成；VPA促进皮质神经元表达GAD，可以使被Met抑制的GAD恢复到正常水平。

    【关键词】 丙戊酸；蛋氨酸；谷氨酸脱羧酶；精神分裂症

    【Abstract】 Objective To study valproate and methionine(Met) regulate glutamic acid decarboxylase (GAD) expression in mouse primary cortical cultures nouron， investigate the mechanism of the VPA’s function in the treatment of schizophrenia.Methods Adopting primary cortical cultures technique cultures neuron, collecte cells, lysis cells, use colorimetric method to measure GAD activities; analyse GAD activities of the vacancy group(serum-free DMEM),the Met group(cultures were incubated with Met at a final concentration of 2 mM in serum-free DMEM for 3days，after that change it with serum-free DMEM)， and the VPA group(cultures were incubated with VPA at a final concentration of 2 mM in DMEM after that incubated with Met at a final concentration of 2 mM in serum-free DMEM for 3days).Results In the Met group the average activities of GAD is obviously lower than the other two group, the difference has statistical meaning. In the VPA group the average activities of GAD is approach to vacancy group ......