淫羊藿;_____淫羊藿;乙醇回流法;提取工艺

正交设计试验法优选淫羊藿提取工艺条件

http://www.100md.com 《广东药学院学报》 2003年第1期

淫羊藿;,,淫羊藿;乙醇回流法;提取工艺,1实验材料,2实验方法,3实验结果,4实验讨论,参考文献

     摘要目的: 优化乙醇回流法提取淫羊藿的工艺条件。方法: 以淫羊藿苷的含量为指标，通过正交设计试验筛选乙醇回流法提取淫羊藿的最佳提取工艺。结果: 影响淫羊藿乙醇回流法提取的主次因素为：溶媒用量>乙醇浓度>提取次数>提取时间,最佳提取工艺条件是: 用8倍80% 的乙醇回流,提取3次,每次30 min。结论: 通过正交设计试验优选得到的淫羊藿乙醇回流法的提取工艺条件稳定可行。

    关键词淫羊藿;乙醇回流法;提取工艺

    Optimization of the extraction process of Herba Epimedii by

    orthogonal design

    WANG Hongrui, YANG Fan ,CHEN Jianyong, TAN Xin， LIANG Huiming

    (1.The Pharmaceutical Section of The Shi Yan People Hospital of Baoan, Shenzhen,Guangdong 518000;

    2.Department of Pharmaceuticals,Guangdong College of Pharmaey,Guangzhou Guangdong 510224)

    Abstract Objective: To optimize the ethanol refluxing and extracting process of Herba Epimedii with orthogonal design. Methods: The optimum extraction conditions were investigated by orthogonal design and with Icariin as the content index. Results: The factors influencing extraction efficiency were as follows: the amount of ethanol > the concentration of ethanol > times of extraction > duration of extraction. The optimum extraction condition obtained was: 80% ethanol,with the volume of 8 folds of Epimedii ,refluxing and extracting for 3 times,30 mins for every turn. Conclusion: The optimized process is feasible for the extraction of Herba Epimedii. ......

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