HPLC法测定复方咖磷颗粒中三种有效组分的含量
维生素B1,,维生素B1；烟酸；咖啡因；反相高效液相色谱法,1仪器与试药,2试验方法与结果,3讨论,参考文献
摘 要 目的 建立同时测定复方咖磷颗粒中维生素B1、烟酸、咖啡因的含量的方法。方法 采用离子对反相高效液相色谱法。 色谱柱：Agilent ZORBAX ExtendC18 (5 μm, 4.6 mm×250 mm), 检测波长为262 nm，线性梯度洗脱。 流动相A：水溶液(0.2 mol/L庚烷磺酸钠溶液1000mL，冰醋酸3.0 mL，用三乙胺调pH7.0)。 流动相B：甲醇。流速：1.0 mL/min。 结果 维生素B1在30.07～90.23 μg/mL，烟酸在3.01～9.04 μg/mL，咖啡因在137.28～411.84 μg/mL范围内呈良好线性关系。r值均为09999，平均回收率分别为99.8%，99.5%，99.6%。RSD分别为0.8%，0.9%，0.6%。结论 本方法简便，快速准确，可一次性测定其中的3种组分。关键词 维生素B1；烟酸；咖啡因；反相高效液相色谱法
HPLC determination of three components in compound caffeine and sodium glycenoposphate granules
YE Xiangyang, ZHOU Guanquan
(Guangzhou Institute for Drug Control,Guangzhou,Guangdong 510160)
Abstract Objective To separate and determine three components in compound caffeine and sodium glycenophosphate granules. Methods RPHPLC was used. Separation was performed on a Agilent ZORBAX extendC18 column (4.6 mm×250 mm, 5 μm). The UVvis detective wavelength is 262 nm and a linear gradient elution is used. Mobile phase A: water solution (1000 mL of 02 mol/L sodium heptanesulfonate, 3.0 mL of acetic acid glacial. Adjust the pH to 7.0 with triethylamine). Mobile phase B: methanol. The flow rate was 1 mL/min. Results It appears to have a good linearity within the concentration range of 30.07~9023 μg/mL for vitamin B1, 3.01~9.04 μg/mL for nicotinic acid and 137.28~411.84 μg/mL for caffeine. The average recoveries are 99.8%, 99.5%, and 99.6% respectively. And the RSD are 0.8%, 0.9% and 0.6%. Conclusion The method is simple, rapid, accurate and reliable. ......
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