淀粉样肽_____淀粉样肽；海马；隔区；神经毒

A β25-35对海马和隔区胆碱能神经元毒性的比较

http://www.100md.com 《广东药学院学报》 2004年第5期

淀粉样肽,,淀粉样肽；海马；隔区；神经毒,Aβ25-35对海马和隔区胆碱能神经元毒性的比较,1材料和方法,2结果,3讨论,参考文献

     摘要目的探讨Aβ25-35对原代培养的海马神经元和隔区神经元毒性作用。方法采用体外原代培养，用Aβ25-35诱导大鼠海马和隔区神经元损伤，MTT法比较两种神经元的存活率，电镜下的形态学变化、并测定SOD活性的改变。结果 Aβ25-35可使海马和隔区神经元的存活率下降，发生凋亡的形态学改变，对两种神经元的MnSOD的活性无明显影响，但可使海马神经元CuZnSOD活性明显下降。结论 Aβ25-35 具有神经毒性，它对两种神经元的损伤程度相似，无细胞差异性，但其毒性损伤作用可能不同。

    关键词淀粉样肽；海马；隔区；神经毒

    Comparison of the effect of βamyloid peptide 25-35 on hippocampal and septal neurons in vitroi

    ZHAO YuHong, HUANG Ren, XU Jie, XIE Yao

    (Section of Pharmacology, Guangdong College of Pharmacy,Guangzhou,Guangdong 510240)

    Abstract Ojbective To study the difference of Aβ25-35 on neurons between hippocampus and septum. Mdthods employing the method of primary cell culture, neurons survival and SOD were examined ,and amalysed the expression of apoptosisrelated gene bclxl by Western blot. Result Aβ25-35 might reduce the survival of hippocampal and septal neurons, increased both activity of CuZnSOD and expression of Bclxl, but decreased the activity of MnSOD of hippocampal neurons, and had on obvious effect on septal neurons. Conclusion Aβ25-35 had the same effect on survival and CuZnSOD but MnSOD between the hippocampus and septum. The cytoxic mechanisms of Aβ25-35 on hippocampus and septus might be different. ......

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