河豚提取物_____河豚提取物；_多肽A；

河豚肝脏提取物多肽A的抗瘤活性实验

http://www.100md.com 《广东药学院学报》 2004年第3期

河豚提取物,,河豚提取物；,多肽A；,抗瘤活性,1材料,2方法,3结果,4讨论,参考文献

     摘要目的：了解河豚肝脏提取物多肽A、B、C的抗瘤活性。方法：体外细胞毒试验是用噻唑蓝(MTT)法。体内抗瘤试验是用小鼠肿瘤模型S180肉瘤。结果：多肽A在125~1 000 mg·L-1浓度下，对人鼻咽癌细胞(CNE2)、人肝癌细胞(Bel7402)、人肺腺癌细胞(GLC82)和人白血病细胞(K562)的半数抑制浓度分别为341.55、607.96、520.82 mg·L-1和833.13 mg·L-1;而对人胚肾上皮细胞(HK293)、人脐血管内皮细胞(EVC403)和人体肝细胞(Chang Liver)的IC50分别为626.22、 57413、493.4 mg·L-1。而多肽B、C对上述细胞的生长均无作用。体内抗瘤试验表明，多肽A用60~180 mg·(kg·d) -1，ip qd×10 d,对小鼠肿瘤S180肉瘤的抑制率为42.0%~45.2% ，与对照组比较差异有显著性(P<0.05)。结论：河豚肝脏提取物多肽A在体内、体外试验有一定的抗瘤活性,但其抗瘤作用与剂量无相关性,对人癌细胞和人体正常细胞无明显的选择性。

    关键词河豚提取物；多肽A；抗瘤活性

    Experimental antitumor activity of polypeptides extracted from hepar of puffer fish

    WANG Honghe，LI Yu, FENG Gongkan, HE Meifeng

    (1. Cancer Institute,Sun YetSen University； 2. Sun YetSenOphthalmic Center, Sun YetSen University, Guangzhou,Guangdong 510060)

    Abstract Objective: to investigate the antitumor activity of polypeptides extracted from hepar of puffer fish. Methods: cytotoxicity test in vitro were measured by MTT assay. In vivo antitumor test used were models of mice S180 sarcoma. Results: under the 125~1000 mg·L-1 of polypeptides,the fifty percent inhibition concentration(IC50) on human nosopharyngeal carcinoma cell line(CNE2), human liver carcinoma cell line(Bel-7402), human lung adenocarcinoma cell line(GlC-82),and human leukemia cell line(K-562) were 341.55 mg·L-1, 607.096 mg·L-1, 520.82 mg·L-1,and 833.13 mg·L-1,respectively . The IC50 value on human embryonic kidney cellline (HEK-293), and Chang Liver cells(CLC), were 626.32 mg·L-1, 574.13 mg·L-1 and 493.4 mg·L-1, respectively. In vivo experiment showed that the tumor inhibitory rates of polypeptides A were 42.0～45.2%(P<0.01), against S-180 sarcoma, under the doses of 60180 mg·(kg·d) -1, intraperitoneal (ip) qd×10d. but polypeptides B and C showed noantitumor effects. Conclusions: the results suggested that the polypeptides A possess antitumor effects in vitro and in vivo, but is not related to doses of polypeptides A, and exhibit noselective inhibition on the human cancer cell and human normal cell. ......

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