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儿童血尿普通光镜检测其形态改变对诊断的意义
http://www.100md.com 《实用医技杂志》 2006年第3期
变形红细胞,,],儿童;普通光学显微镜;相差显微镜;变形红细胞,1材料与方法,2血尿红细胞形态和数量诊断标准,3结果,4讨论,参考文献:
     [摘 要] 目的:在实验室用普通光学显微镜取代相差显微镜,检测尿中红细胞形态改变对儿童血尿来源诊断具有实际意义。方法:留取晨尿50 ml混匀,取10 ml倒入刻度管内。1 500 r/min,5 min弃上清留0.2 ml。滴在载玻片上。18 mm×18 mm盖玻片压住。10×40的镜头检查。结果:表明有2种以上形态变化的红细胞占尿红细胞80%以上即可诊断为肾小球性血尿。20%~80%之间为混合型血尿。20%以下为非肾性血尿。结论:用普通光学显微镜替代相差显微镜检查尿中红细胞形态鉴别其来源。方法和操作准确简便易行。

    [关键词] 儿童;普通光学显微镜;相差显微镜;变形红细胞

    Child Hematuria Ordinary Light Mirror Examines Its Shape Change the Significance

    ZHANG Li,HU Xuefeng

    (Childrens Hospital of Changchun Changchun, Jilin 130051,China)

    Abstract:Objective In the laboratory with the ordinary light microscope substitution phase contrast microscope,in the examination urine the red blood cell shape change originates the diagnosis to the child hematuria to have the practical significance. Methods Is it fetch urine 50 ml mix even to fetch 10 ml pour scale into in the test tube fresh morning to leave.1 500 r/min.5 min abandons and leavesclearly 0.2 ml.Drip on glass slice of year.18 mm×18 mm is covered the glass slice and pushed down.10×40 lenses are checked.Results Is it have 2 more than red blood corpuscles that shape change account for urine 80% more than red blood corpuscles namely diagnosable for kidney little ball blood in the urine to indicate.For the mixing type blood in the urine between 20%~80%.20% of the the following is not the kidney blood in the urine. ......

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