乙醇提取物_____]地椒;

中药地椒乙醇提取物对人白血病细胞增殖的抑制作用

http://www.100md.com 《中西医结合学报》 2005年第5期

乙醇提取物,,]地椒;,乙醇提取物;,乙酸乙酯萃取物;,抗肿瘤药;,K562;,HL60,1材料与方法,2结果,3讨论,[参考文献]

     [摘要]目的：筛选中药地椒乙醇提取物的抗肿瘤活性成分，探讨其可能的抗肿瘤作用机制。方法：从野生地椒乙醇提取物中分离得到乙酸乙酯、正丁醇和丙酮3种萃取组分，以体外培养的人白血病细胞株K562和HL60作为研究对象。将各萃取组分与肿瘤细胞作用一定时间后，计数存活肿瘤细胞数，分析药物对肿瘤细胞生长曲线的影响，考察各组分对肿瘤细胞生长的抑制作用。以去甲斑蝥素作为阳性对照组，通过形态学分析等方法，研究中药地椒乙醇提取物可能的抗肿瘤作用机制。结果：中药地椒乙酸乙酯萃取物对人白血病细胞株K562和HL60均有显著的抑制作用，且呈药物浓度依赖性，并可诱导肿瘤细胞凋亡；而正丁醇和丙酮萃取物对两种白血病细胞均无显著的抑制作用。结论：体外实验表明，乙酸乙酯萃取物是中药地椒乙醇提取物中主要的抗肿瘤活性成分。

    [关键词]地椒; 乙醇提取物; 乙酸乙酯萃取物; 抗肿瘤药; K562; HL60

    Antitumor effect of ethanol extracts from Thymus quinquecostatus Celak on human leukemia cell line

    SUN ZhenXiao, ZHANG YingHui, CHENG Shuang, MA QingWen, GUO ShanLi, ZHANG JinBao

    (School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China; College of Life Science, Liaocheng University, Liaocheng, Shandong Province 252059, China)

    ABSTRACT Objective: To screen the antitumor fraction of ethanol extracts from Thymus quinquecostatus Celak and investigate its antitumor effect on human leukemia cell line. Methods: Ethyl acetate, nbutanol and acetone fractions were separated from the ethanol extracts of wild Thymus quinquecostatus Celak. Growth inhibiting effects of these extracts on human leukemia cell lines K562 and HL60 were determined by live cell counting and cell growth curve analysis. The possible antitumor mechanism was studied by morphological analysis with norcantharidin as a positive control. Results: Ethyl acetate fraction could significantly inhibit the proliferations of K562 and HL60 cells, and the inhibiting effect depended on the concentration of ethyl acetate fraction. Ethyl acetate fraction could induce apoptosis of K562 and HL60 cells. The nbutanol and acetone fractions had no significant inhibiting effect on K562 and HL60 cells. Conclusion: Ethyl acetate fraction is the major antitumor fraction in ethanol extracts from Thymus quinquecostatus Celak. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/31/98/29/34.htm

您现在查看是摘要页，全文长 11810 字符。