膀胱癌肿瘤 睾丸抗原相关基因的表达
膀胱肿瘤,,膀胱肿瘤;肿瘤睾丸抗原;MAGEA1;,SSX2;,NYESO1;,MAGEC1;MAGEE1;HAGE,膀胱癌肿瘤睾丸抗原相关基因的表达,0引言,1材料和方法,2结果,3讨论
Expression of cancer/testis antigenrelated genes in human bladder cancerYAN HongLiang, WANG He, LI Xin, LIU HeLiang
Department of Urology Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To investigate the genes expression of cancer/testis antigens (CTA) in human bladder cancer. METHODS: Reversetranscription polymerase chain reaction (RTPCR) was used to investigate the expression of the MAGEA1, SSX2, NYESO1, MAGEC1, MAGEE1 and HAGE genes in 33 bladder cancer samples and corresponding nontumorous bladder tissues. Three samples randomly selected from each CTA PCR product were sequenced. RESULTS: In the 33 tumor samples, the MAGEA1, SSX2, NYESO1, MAGEC1, MAGEE1 and HAGE mRNA expression rates were respectively 21.2% (7/33), 39.4% (13/33), 69.7% (23/33), 42.4% (14/33), 36.4% (12/33) and 24.2% (8/33). In tumor tissues, the positivity rate was found to be 75.8% (25/33) for at least one of these genes expression, and 69.7% (23/33) for two and more than two. Almost none of the nontumorous bladder tissues were positive for these genes. The DNA sequence confirmed that the RTPCR products were CTA cDNA. CONCLUSION: The cancer/testis antigens are potential targets for antigenspecific immunotherapy of bladder cancer. The coexpression pattern of these antigens provides a theoretic foundation for the development of a polyvalent bladder cancer vaccine. ......
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