Human multiª²tissue expression profile induced by ILª²6 and functional forecast of novel gene LX3

    XIA Rong1, LI Yan2, ZHANG Jiª²Yan2, FENG Jianª²Nan2, SHEN Beiª²Fen2

    1Department of Blood Transfusion, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China, 2Institute of Basic Medical Science, Academy of Military Medical sciences, Beijing 100850, China

    ¡¾Abstract¡¿ AIM: To study the expression status of novel gene LX3 induced by ILª²6 in human multiple tissues and to analyze LX3 protein superfamily so as to forecast the function of the novel gene. METHODS: Full length LX3 cDNA probe tagged by 32P was synthesized by means of APª²PCR. The expression of novel gene LX3 on multiple tissue RNA blots was analyzed (MTRB) by the method of Northern Blot. To obtain more functional information, we carried out further analysis of LX3 protein superfamily. RESULTS: The novel gene LX3 was expressed, to different degrees, in 8 human tissues, with strong expressiion in the spleen and spermary but weak in the brain and stomach. Superfamily analysis confirmed that LX3 protein shared the high homology with the protein named 2GBP, a member of periplasmic binding protein. CONCLUSION: There is a high correlation between LX3 and lymphocyte proliferation induced by ILª²6. ......