GSTGankyrin融合蛋白的原核表达及抗体制备
融合蛋白,,Gankyrin;GST融合蛋白;多克隆抗体,0引言,1材料和方法,2结果,3讨论,【参考文献】
Expression of GankyrinGST fusion protein in E.coli and preparation of its specific polyclonal antibodyYANG ZhaoXu1, DOU KeFeng1, LU Fan2, ZHAO ZhongLiang2
1Department of Hepatobiliary Surgery, Xijing Hospital, 2Department of Biochemistry and Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To express Gankyrin in E. coli and to prepare antibody against it. METHODS: The core fragment of Gankyrin was cloned into plasmid pGEX4T2 containing glutathione stransferase (GST) fusion protein gene. Following the restriction enzyme digestion analysis and sequencing, pGEX4TGankyrin was transformed into E. coli DH5α. GSTGankyrin fusion protein was expressed under IPTG induction and the Gankyrin protein was used to immunize New Zealand rabbits. The specific antibody against Gankyrin was identified by ELISA and Western blot. RESULTS: GSTGankyrin fusion protein was expressed in E.coli and its specific polyclonal antibody was obtained. CONCLUSION: The successful expression of GSTGankyrin fusion protein in E.coli and the preparation of its specific polyclonal antibody will facilitate the study of the biological functions of Gankyrin. ......
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