骨关节炎_____骨关节炎；_威灵仙注射液；

威灵仙注射液对骨关节炎模型动物软骨组织形态和胶原表达的影响

http://www.100md.com 《中国矫形外科杂志》 2006年第5期

骨关节炎,,骨关节炎；,威灵仙注射液；,组织学；,Ⅱ型胶原；,超微结构,1材料和方法,2结果,3讨论,参考文献：

     摘要：[目的]探讨威灵仙注射液对骨关节炎模型动物关节软骨的组织学、胶原表型和超微结构的作用。[方法]建立骨关节炎动物模型，动物随机分为3组，S组、D组和K组，S组和D组分别注射威灵仙注射液和生理盐水，K组空白。分批处死动物取关节软骨标本，常规石蜡切片，HE/SOFC双染色，Mankin评分比较各组关节软骨损伤程度。免疫组化法(IHC)检测各组关节软骨基质Ⅰ、Ⅱ型胶原的表达情况。透射电镜(TEM)观察各组关节软骨超微结构变化。[结果]S组在各时段的评分均优于D组和K组；S组在各时点Ⅰ型胶原阳染面积低于D组和K组，Ⅱ型胶原阳染面积高于D组和K组；S组软骨在各时间点损伤程度都低于D组和K组，K组和D组损伤程度基本一致。[结论]威灵仙注射液可维持和促进软骨合成蛋白多糖与Ⅱ型胶原，对关节软骨具有保护作用。

    关键词：骨关节炎；威灵仙注射液；组织学； Ⅱ型胶原；超微结构

    Effect of sixpetal clematis root injection on histomorphology and collagen expression in cartilage of osteoarthritis animal model∥CHEN Feiyan,WANG Xu,HUANG Jiazhang,et alOrthopedic Department,Affiliated Huashan Hospital,Fudan University,Shanghai 200040

    Abstract：[Objective]To study the effect of sixpetal clematis root injection(WLX)on histology,collagen phenotype and ultramicrostructurc in cartilage of osteoarthritis animal model[Method]OA model animals were randomly divided into 3 groups:group S,group D and group KAnimals in group S received WLX and animals in group D received sterilized normal saline intramuscularly,group K was control and received nothingAfter the animals were killed,cartilage specimen were obtained and sections were made and stained with HE/SOFG for Mankin score;the expression level of collagen Ⅰ and Ⅱ in matrix was tested by immunohistochemistry;change of ultramicrostructure of cartilage was observed with transmission electron microscope[Result]Mankin score in group S was better than that in group D and K at each test period;result of IHC shows that positive stain area of collagen Ⅰ was lower in group S than that in group D and K,and positive stain area of collagen Ⅱ was higher than that in group D and K at each test period;cartilage impairment degree in group S was lower than in group D and K,and impairment manifestation in group D and K was similar on the whole[Conclusion]sixpetal clematis root injection may protect articular cartilage by maintaining and promoting the chondrocyte synthesizing proteoglycan and type Ⅱ collagen ......

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