Antiª²HBV effect of siRNA expression plasmid targeting the S antigen of HBV

    ZHANG Yan,BAI Xueª²Fan,ZHANG Ying,HUANG Changª²Xing,LI Yu,LI Guangª²Yu,WANG Pingª²Zhong,HONG Sa,CHEN Weiª²Hong,REN Guangª²Li

    Center of Infectious Diseases of Chinese PLA,Tangdu Hospital,Fourth Military Medical University,Xi¬ðan 710038,China

    ¡¾Abstract¡¿ AIM£º To identify the siRNA interference ability for the replication of HBV. METHODS£º Based on the sequence of HBV in GenBank, siRNA targeting the Sª²RNA of HBV were synthesized and cloned into pSilencer ZY1, pSilencer ZY2, pSilencer ZY3 and pSilencer HK. Transfected into HepG2 2ª±2. 15 cells with the new recombinant vectors was carried out by Metafectene . The expression of HBsAg and HBeAg was assayed by MEIA and HBV Sª²mRNA was detected by RTª²PCR. RESULTS£º The construction of the recombinant expression vectors was successfully conformed by the results of enzyme digestion, electrophoresis and gene sequencing. The three siRNAs could suppress HBV Sª²mRNA and the expression of HBsAg and HBeAg in the supernatant of HepG2 2ª±2ª±15 cells. The inhibitory rates of HBsAg were 81%, 29% and 78%, and that of HBeAg were 35%, 3% and 49% respectively. CONCLUSION£º The three siRNA mentioned above targeting the Sª²mRNA of HBV could effectively inhibit HBV replication. ......