阿尔茨海默病__(期刊论文)

Humanin的基因克隆及序列测定

http://www.100md.com 《西安交通大学学报》 2006年第2期

阿尔茨海默病,,阿尔茨海默病；Humanin；基因克隆；序列测定,1材料与方法,2结果,3讨论,参考文献：

     作者简介：靳辉(1973)，女(汉族),解剖学硕士. 研究方向：阿尔茨海默病的病理机制及防治.

    摘要：目的利用基因工程方法对Humanin进行基因克隆并测定其序列，为进一步对阿尔茨海默病进行基因治疗奠定基础。方法采用非对称互补引物/模板法，制备两端含有酶切位点的Humanin的cDNA，将其克隆入pVAXI载体中并测序。结果经酶切鉴定、测序分析，表明所插入的基因片断为Humanin基因，与设计完全相同。结论成功地克隆了Humanin基因。

    关键词：阿尔茨海默病；Humanin；基因克隆；序列测定

    The cloning and sequencing of HumaninJin Hui, Wang Weixi, Yang Guangxiao, Wang Quanying, Hu Haitao, Han Taizhen

    (1. Department of Human Anatomy, Histology and Embryology, Medical School of Xian Jiaotong University, Xian 710061;

    2. Xian Huaguang Biological Engineering Ld Company, Xian 710025;

    3. Department of Physiology and Pathophysiology, Xian Jiaotong University, Xian 710061, China)

    ABSTRACT: Objective To use gene engineering technique to clone and sequence theHumanin. Methods By means of asymmetrical primer/template, double stranded cDNA of Humanin was constructed, which had restriction enzymes sites on the two extremes. Then the cDNA was subcloned into the plasmid pVAXI and sequenced. Results Evidences of DNA sequence analysis and restriction enzymes digestion showed that the inserted fragment wasHumanin cDNA, consistent with the fragment we designed. Conclusion The Humanin cDNA was cloned successfully. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/06/10/00/74/13.htm

您现在查看是摘要页，全文长 8648 字符。