动静力失衡_____动静力失衡；_椎间盘；

动静力失衡性大鼠颈椎间盘组织形态学及超微结构

http://www.100md.com 《中国矫形外科杂志》 2006年第5期

动静力失衡,,动静力失衡；,椎间盘；,形态学；,超微结构；,动物模型,1材料与方法,2结果,3讨论,参考文献：

     摘要：[目的]动态观察大鼠颈部动、静力失去平衡后颈椎间盘组织形态学及超微结构的改变。[方法]SD大鼠60只，随机分为3、5、7个月模型组和对照假手术组，每组10只，通过破坏大鼠颈部肌肉、韧带诱导颈椎间盘退变，按月取材。各组椎间盘HE、Toluidine blue染色，在光镜下观察用体视学分析软件、Dissector自动计数法分析软骨终板内血管芽的数量、面积和软骨终板的厚度，Miyamoto分级法综合评判颈椎间盘退变的程度；透射电镜观察椎间盘细胞凋亡的超微结构。[结果]与对照假手术组比较，3个月造模组动物颈椎间盘已开始退行性变化，纤维环板层结构紊乱，关节软骨钙化层增厚，软骨下血管明显减少；5个月模型组髓核完全纤维化，纤维环板层状结构消失，血管芽稀少，血管壁充血曲张；7个月模型组椎间盘内部结构与5个月模型组相似，部分椎体边缘骨赘形成。在电镜下，3个月造模组椎间盘细胞，细胞表面突起减少，细胞器稀少，胞质内有脂滴，可见到凋亡细胞形成的凋亡小体。5个月和7个月模型组椎间盘组织中细胞较少，基质中胶原纤维断裂，可见到许多坏死空化的细胞。[结论]模型组大鼠颈椎间盘都出现典型的退变形态学变化，随着造模月份的延长退变程度越严重；早期和中期退变椎间盘中可观察到凋亡细胞，在退变的晚期可观察到坏死细胞。

    关键词：动静力失衡；椎间盘；形态学；超微结构；动物模型

    Histomorphology and ultrastructure in the rat model with cervical vertebral unbalance of dynamic and static force∥LI Chenguang,WANG Yongjun,SHI Qi,et alInstitute of Spine,Longhua Hospital,Shanghai University of TCM,Shanghai 200032

    Abstract：[Objective]To observe the histomorphology and ultrastructure of the intervertebral disc ( I D ) in the rat model with cervical vertebral unbalance of dynamic and static force[Method]Sixty SD rates were randomly divided into 4 groups: 3 months,5 months,7 months and the control groupThe cervical I D degeneration model was made by destroying the neck muscle of rats and the tissues wer collected every monthThe number,area and thickness of the cartilage endplate were measured by the means of Miyamoto's ClassesThe ultrastructure of the apoptosis cells of the intervertebral disc was observed under the electron microscope[Result]Compared with the control group,the 3 months group showed degeneratin changes,with disordered structure of the annular fibrosis and thickness increase of calcification layer and decrease of blood vessel unmber in the cartilage layerComplete fibrosis was found in nucleus pulposus in 5 months model group,with the fibro lamellar structure disappeared and few blood vessel budsThe features of 7 months model group was similar to 5 months and osteophyma had been formed near the bordr of part intervertebalUnder the electron microscope,the number of surface projection and organelles was decreasedFatty drop and apoptotic body could be seen in disc cells of 3 month model groupFew cells,broken collagen fibers in ECM and more cavitation cells for necrosis in 5 months and 7months model groups could be seen[Conclusion]The cervical I D of model groups has shown typical morphological changes of degeneration and this trend was more serious with the time passingIn the early and middle stage of degeneration,the apoptosis cells can be seen,but in the terminal stage the cellular necrosis was more common ......

百拇医药网 http://www.100md.com/html/DirDu/2006/06/10/00/77/46.htm

您现在查看是摘要页，全文长 14540 字符。