【摘要】 目的 异黄酮类化合物无光或很弱，光化学反应后会产生光，但大多仍不强。本文探索尿素对异黄酮作用，为异黄酮类化合物的分析提供微量方法。方法 在乙醇中加尿素-HCl液再加0.1～1μg异黄酮，经UV光照后，测光。结果 发现尿素与13个异黄酮经光化学反应后有8个可增强光，5个化合物无增强作用。结论 尿素与异黄酮光化学反应后光增强的化合物其R4必需是氨基或甲氧基或甲氧基中一个氢被取代物。R4为-OH，-NO2取代物无作用。异黄酮的R4是OCH3、OCH2R，NH2基团的化合物在酸中与尿素经光化学反应可增加光，达到微量分析的要求。

    【关键词】 尿素；异黄酮；光化学反应；低温光

    【Abstract】 Objective Isofavons do not phosphorescence or very low.It phosphoresces after UV irradiation.but it is still not strong enough.For the purpose of clinical and pharmaceutical analysis it is necessary to develop a new sensitive method for microanalysis.Methods The solution of urea-HCl were added to the ethanol solution of isoflavons and measured the phosphorescence after UV irradiation.Results An intensified phosphorescence of isoflavons were obtained after treatment.Among 13 isoflavons tested ......