[摘要]目的 筛选并鉴定与表达人CD59的中国仓鼠卵巢(CHO)细胞特异结合的内化短肽，为设计具有拮 抗CD59肿瘤逃逸活性的短肽药物奠定基础。 方法 以高表达人CD59的CHO细胞为靶，对噬菌体随机十二肽库 进行5轮亲和筛选，并进行竞争结合实验，用ELISA法筛选噬菌体阳性克隆并对其进行DNA序列分析。 结果 随机挑选的10个克隆中有6个与人CD59结合力高，测序后得到2个高度同源的氨基酸序列。 结论 获得了与高 表达人CD59的CHO细胞结合的内化短肽序列，为进一步设计与肿瘤逃逸相关的CD59活性位点的短肽药物提供 了参考依据。

     [关键词]卵巢细胞;肽库;内化短肽;CD59蛋白;膜蛋白质类

    SCREENING THE SHORT INTERNATION PEPTIDE COMBINED WITH CHO CD59 THROUGH PHAGE

    CHENG YING，GAO MEI-HUA ， WANG BING， et al

    (Department of Immunology， Qingdao University Medical College ，Qingdao 266021， China)

    [ABSTRACT]Objective To search for a short internation peptide specifically combined with CHO cells for the purpose to establish a base of designing a peptide drug to inhibit CD59 on tumor sneaking through. Methods Taking CHO cells of high-ex- pressing CD59 as a target，a five-round affinity screening was done randomly for peptide library 12. After competitive test， positive phage clones were selected by ELISA and subsequently underwent DNA sequencing. Results Six out of 10 selected phage clones possessed the high affinity to CD59. After DNA sequencing ......