辛伐他汀_____辛伐他汀;_破骨细胞;

辛伐他汀对PTHrP诱导的破骨细胞性骨吸收及小鼠颅盖骨代谢的影响

http://www.100md.com 《中国矫形外科杂志》 2006年第9期

辛伐他汀,,辛伐他汀;,破骨细胞;,骨吸收;,甲状旁腺素相关肽(PTHrP),摘要,关键词,1材料和方法,2结果,3讨论,参考文

     摘要：[目的]探讨辛伐他汀对体外甲状旁腺素相关肽(PTHrP)诱导小鼠的破骨细胞骨吸收功能的作用及其小鼠骨代谢的影响。[方法]采用PTHrP诱导小鼠骨髓细胞培养破骨细胞和小鼠颅盖骨培养体系，检测辛伐他汀作用8 d后破骨细胞骨吸收陷窝和培养上清钙的变化；检测小鼠颅盖骨培养上清碱性磷酸酶和钙含量，组织学观察小鼠颅盖骨形态学变化。[结果]辛伐他汀体外可明显抑制PTHrP诱导小鼠的破骨细胞骨吸收陷窝的形成及培养上清钙的释放，辛伐他汀体外可增强小鼠颅盖骨培养上清碱性磷酸酶的活性，组织学观察到辛伐他汀使小鼠颅盖骨矿化增强。[结论]辛伐他汀体外不仅可促进小鼠颅盖骨的成骨活性，并且可明显抑制PTHrP诱导小鼠的破骨细胞骨吸收功能，对骨吸收性疾病有着重要的防治作用。

     关键词：辛伐他汀; 破骨细胞; 骨吸收; 甲状旁腺素相关肽(PTHrP)

    Effect of simvastatin on the osteoclastic resorption stimulated by PTHrP and anabolism with murine calvarial organ culture in vitro∥HUANG Luyu,HU Yunyu,TAO Huiren,et alDepartment of Orthopaedics,Xijing Hospital,the Forth Military Medical University,Xian 710032

    Abstract：[Objective]To study the effect of simvastatin in the osteoclastic resorption stimulated by PTHrP and murine bone anabolism in vitro[Method]The bone resorption activities of the osteoclast stimulated by PTHrP were evaluated after treatment with simvastatin for 8 days in vitro;the concentration of Ca2+ in the supernatant was also detected by atomic absorption spectrometerThe concentration of ALP and Ca2+ of the supematant in murine calvarial organ culture were detectedThe histology of calvaria was observed[Result]Simvastatin greatly inhibited the osteoclastic bone resorption stimulated by PTHrP in vitro and reduced the release of Ca2+Simvastatin increased the ALP activities and bone mineralization of murines calvarial organ culture in vitro[Conclusion]Simvastatin may inhibit the osteoclasric resorption stimulated by PTHrP and promote osteoblast differentiation and bone mineralization in vitro,thus play an important role in the prevention and treatment of osteoporosis ......

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