SMMC-7721肝癌细胞膜上β2糖蛋白Ⅰ受体的表达
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高沿航, 高普均, 王 丹, 时 阳, 李玉琴,
肝癌;SMMC-7721细胞系;β2糖蛋白Ⅰ;乙型肝炎病毒;受体;流式细胞术,SMMC-7721肝癌细胞膜上β2糖蛋白Ⅰ受体的表达,高沿航,高普均,王丹,时阳,李玉琴,朴云峰,杨翰仪,通讯作者,Exp
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高沿航, 高普均, 王丹, 时阳, 李玉琴, 朴云峰, 吉林大学第一医院消化内科 吉林省长春市 130021
杨翰仪, 吉林大学基础医学院生物化学教研室 吉林省长春市 130021
国家自然科学基金资助项目, No. 30572106
通讯作者: 高普均, 130021, 吉林省长春市, 吉林大学第一医院消化内科. pujun-gao@163.com
电话: 0431-5612162
收稿日期: 2006-02-13 接受日期: 2006-04-30
Expression of beta-2-glycoprotein Ⅰ receptor on membranes of hepatoma cell line SMMC-7721
Yan-Hang Gao, Pu-Jun Gao, Dan Wang, Yang Shi, Yu-Qin Li, Yun-Feng Piao, Han-Yi Yang
Yan-Hang Gao, Pu-Jun Gao, Dan Wang, Yang Shi, Yu-Qin Li, Yun-Feng Piao, Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China
Han-Yi Yang, Department of Biochemistry, School of Basic Medicine, Jilin University, Changchun 130021, Jilin Province, China
Supported by National Natural Science Foundation of China, No. 30572106
Correspondence to: Dr. Pu-Jun Gao, Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China. pujun-gao@163.com
Received: 2006-02-13 Accepted: 2006-04-30
Abstract
AIM: To further study the interaction between beta-2-glycoprotein Ⅰ(β2GPⅠ) and the membrane of hepatoma SMMC-7721 cells, and to explore the mechanism of hepatitis B virus (HBV) infection.
METHODS: Fluorescence microscopy and fluorescence activated cell sorter (FACS) was used to observe the interaction of β2GPⅠ with the hepatoma cell line SMMC-7721, gastric carcinoma cell line SGC-7901 and lymphoma cell line HL-60.
RESULTS: Fluorescence microscopy revealed specific binding of FITC-β2GPⅠ to SMMC-7721 cells, but neither to HL-60 nor SGC-7901 cells. FACS analysis demonstrated that the binding rate of FITC-β2GPⅠ (20 μL) to SMMC-7721 cells was significantly higher than that in HL-60 or SGC-7901 cells (19% vs 1.7%, 1.9%, both P < 0.01). When 50 μL FITC-β2GPⅠ was used, the binding rate of FITC-β2GPⅠ to SMMC-7721 cells was 20 ......
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