DNA甲基化对大肠癌相关基因表达的调控意义
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李 庚, 千新来, 崔 静, 王中群, 冶亚平,
大肠癌;DNA甲基化;p16;Rb;cyclinD1,李庚,千新来,崔静,王中群,冶亚平,杨晓煜,李永真,李庚,通讯作者,RoleofDNAmethylat
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李庚, 千新来, 崔静, 王中群, 冶亚平, 杨晓煜, 李永真, 新乡医学院病理教研室 河南省新乡市 453003
李庚, 1995年新乡医学院学士, 2002年山西医科大学获病理学硕士, 讲师, 主要从事肿瘤的病理研究.
河南省科技发展计划基金资助项目, No. 0124170620
通讯作者: 千新来, 453003, 河南省新乡市, 新乡医学院病理教研室. qxlfssws@163.com
电话: 0373-3029123
收稿日期: 2006-03-14 接受日期: 2006-05-08
Role of DNA methylation in control of tumor suppressor gene and oncogene expression in colorectal carcinoma
Geng Li, Xin-Lai Qian, Jing Cui, Zhong-Qun Wang, Ya-Ping Ye, Xiao-Yu Yang, Yong-Zhen Li
Geng Li, Xin-Lai Qian, Jing Cui, Zhong-Qun Wang, Ya-Ping Ye, Xiao-Yu Yang, Yong-Zhen Li, Department of Pathology, Xinxiang Medical College, Xinxiang 453003, Henan Province, China
Supported by the Foundation from Scientific Technology Development Plan of Henan Province, No. 0124170620
Correspondence to: Dr. Xin-Lai Qian, Department of Pathology, Xinxiang Medical College, Xinxiang 453003, Henan Province, China. qxlfssws@163.com
Received: 2006-03-14 Accepted: 2006-05-08
Abstract
AIM: To investigate the relationship between DNA methylation and expression of p16, Rb, and cyclin D1 in the carcinogenesis of colorectal carcinoma.
METHODS: Methylation-specific polymerase chain reaction (MS-PCR) was used to detect the methylation status of p16, Rb, and cyclin D1 in the specimens from colorectal carcinoma, cancer-adjacent tissues of carcinoma and adenoma, and normal colorectal mucosa, respectively. The correlations of methylation with protein expression and the clinicopathological indexes were analyzed.
RESULTS: For the levels of p16 and Rb methylation, there were increased tendencies in the carcinogenesis of colorectal cancer, while for the level of cyclin D1 methylation, there was a decreased tendency. The expression of p16 and cyclin D1 protein were inversely correlated with the methylation status of p16 (normal mucosa:r = -0 ......
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