抑癌基因__

抑癌基因nm23H1对化疗药物增敏作用的体外实验研究

http://www.100md.com 《广东药学院学报》 2002年第2期

抑癌基因,,抑癌基因；nm23H1；化疗药物；增敏作用；体外实验,1材料与方法,2结果,3讨论,参考文献

     摘要目的：探讨抑癌基因nm23H1对几种常用化疗药物的增敏作用。方法：构建含有人全长的nm23H1基因cDNA的真核表达载体质粒pcDNA3nm23H1，通过脂质体介导，将它和空载体质粒pcDNA3分别转染鼠肺腺癌LA795细胞株中，得到的阳性转染子分别命名为pcDNA3nm23H1795细胞、pcDNA3795细胞，另设795细胞为正常对照组。用MTT法测定nm23H1对顺铂、氮芥、丝裂霉素C、5-氟尿嘧啶、长春新碱和足叶乙苷的增敏效应。结果：nm23H1基因在pcDNA3nm23H1795细胞中获得了表达，MTT法检测nm23H1对顺铂、氮芥、丝裂霉素C有不同程度的增敏作用，而对5-氟尿嘧啶、长春新碱和足叶乙苷没有此效应。结论：nm23H1可增加化疗药物顺铂、氮芥、丝裂霉素C对LA795细胞的敏感作用。

    关键词抑癌基因；nm23H1；化疗药物；增敏作用；体外实验

    Effect of tumor suppressor gene nm23H1 in enhancing sensitivity of mice lung carcinoma cell to chemotherapeutic agents

    Abstract Objective: To investigate the sensitivity enhancing effect of tumer suppressor gene nm23H1 to chemotherapeutic agents. Methods: Recombinant eukaryotic expression vector pcDNA3nm23H1 containing full length human nm23H1 cDNA was constructed and then transfected into mice lung carcinoma cell line LA795. Sensitivites of the cell lines to cisplatin, mechlorethamine, mitomycin C, 5-fluorouracil, vincristine and vp16 were determined by MTT assay. Results: nm23H1 gene was successfully transfected into 795 cells and highly expressed. MTT assay showed that nm23H1 could enhance the sensitivity of the cell line to cisplatin, mechlorethamine and mitomycin C but not to 5-fluorouracil, vincristine and vp16. Conclusions: nm23H1 enhances the sensitivity of LA795 cell line to some chemotherapeutic agents. ......

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