抗癫痫药_____抗癫痫药;_HPLC;

反相HPLC法同时测定多种抗癫痫药物的血清浓度

http://www.100md.com 《广东药学院学报》 2002年第1期

抗癫痫药,,抗癫痫药;,HPLC;,血药浓度,1仪器及试剂,2实验方法,3结果,4讨论,参考文献

     摘要目的: 建立同时测定血清中多种抗癫痫药物浓度的方法。方法:血清用叔丁基甲醚(MTBE)两次提取, 室温下N2吹干, 残渣用甲醇溶解, 进样分析, 色谱条件为C18柱(250 mm×4.6 mm,5 μm), 流动相为ψ(甲醇∶乙腈∶水)=40∶13∶47, 检测波长240 nm。结果: 7种药物一次性分离, 血清杂质不干扰测定, 线性方程相关系数大于0.999, 回收率为92%～105%。结论:方法专属性好，适用于临床血药浓度监测及药物的定性定量分析。

    关键词抗癫痫药; HPLC; 血药浓度

    Simultaneous determination of antiepileptics drugs in serum by HPLC

    HAN Liping, LUO Ke, ZHANG Yanjun

    (General Hospital of Guangzhou Military Command,Guangzhou,Guangdong 510010)

    Abstract Objective：To establish a rapid and simple method for the determination of antipileptic drugs in serum simultaneously. Methods: The serum samples were extracted twice by MTBE. The residue was reconstituted in methanol and detected at 240 nm.The mobile phase consisted of methanolacetonitrilewater (40∶13∶17).The YWC18(250 mm×4.6 mm,5 μm) column was used. Results: 7 drugs were well separated. The correlation coefficient of the linear equation was larger than 0.999. The recoveries were from 92% to 105%, and the variation coefficients of the betweenday and withinday assay were less than 3%. Conclution: The method was simple,rapid and specific. It was suitable for qualitative and quantitative determination of toxic drugs in serum simultaneously. ......

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