Establishment of delayed neuropsychologic sequelae rat model after acute carbon monoxide poisoning

    ZHAO Tianª²Zhi, CHANG Yaoª²Ming, LI Jinª²Sheng, XIE Xiaoª²Ping

    Department of Aerospace Hygiene and Health Service, School of Aerospace Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To establish the delayed neuropsychologic sequelae rat model after acute carbon monoxide (CO) poisoning for further elucidating its pathogenesis. METHODS: Male SD rats (240-280 g BW) were poisoned with CO in the animal experimental hyperbaric oxygen chamber. Cognitive ability was measured by the escape latency of searching the objective in the Morris water maze. The brains of the rats (3, 7, 10, 20 d after poisoning) were taken for paraffin pathological section and HE staining to observe the pathological change in cerebral cortex and hippocampal area. RESULTS: Rats lost consciousness when they were exposed to 2000 ppm (part per million) CO for 40 min and then 3000 ppm for up to 20 min. Maze test showed that the escape latency of the CO poisoned rats was significantly longer than the air exposed rats (F=4.74 ......