【摘要】 目的 研究弓形虫感染对卵巢功能的影响。方法 将雌性小鼠随机分为实验组和对照组，每组各30例，实验组在雌性小鼠感染弓形虫后第45、90天的发情间期采血，对照组也在相应的时期采血。采用放射免疫法测定血清中雌二醇(E2)含量，并于完成两次采血后取两组小鼠卵巢和脑组织送病理检查。结果 实验组45天时血清E2为(12.37±5.56)pg/ml，90天时为(7.98±4.59)pg/ml；对照组45天时血清E2为(10.99±8.71)pg/ml，90天为(10.85±5.06)pg/ml；实验组90天时E2水平下降，与45天时及对照组比较，差异均有显著性(P<0.05)。实验组小鼠脑组织见慢性炎症改变及弓形虫包囊体；卵巢呈炎性改变；对照组小鼠脑组织和卵巢未见异常。结论 弓形虫感染可以导致小鼠卵巢功能下降。

     【关键词】 弓形虫；卵巢疾病

    Effects of toxoplasma infection on ovarian function in mice

    ZHENG En-lan,LUO Ruo-yu.Department of Obstetrics and Gynecology,The 2nd People's Hospital,Xinzhou District,Wuhan 430415,China

    【Abstract】 Objective To study the effects of toxoplasma infection on ovarian function in Kunming female mice.Methods The Kunming female mice were devided to two groups (each n=30):experiment group were infected with AH strain of toxoplasma,and control group were not infected with AH strain of toxoplasma.After 45 days and 90 days,the blood were collected in the estrum interphase,and estrogen in serum were detected by radioimmunoassay.The histology of the brain and ovary were observed by microscope.Results The level of estrogen in serum of experiment group was (12.37±5.56)pg/ml at 45 days,and (7.98±4.59)pg/ml at 90 days;The level of estrogen in serum of not infected group was (10.99±8.71)pg/ml at 45 days,and (10.85±5.06)pg/ml at 90 days.The level of estrogen in serum of experiment group at 90 days was reduced significantly compared with the level at 45 days and control group(P<0.05).The histology of the brain was phlogistic change and found Toxoplasma cysts body,and histology of the ovary was phlogistic change in experiment group;the histology of the brain and ovary in control group was not found abnormal.Conclusion The infection of toxoplasma possibly reduced the ovarian function in KM female mice. ......