【摘要】 目的 建立动物心肌缺血模型，探讨心肌缺血时细胞骨架的改变。方法 15只清洁级健康成年雄性Wistar大白鼠，采用左冠状动脉结扎术造成部分心肌缺血，分别于缺血30min、60min、120min时取缺血心肌组织用免疫组织化学方法观察心肌细胞骨架蛋白肌动蛋白(α-actin)、波形蛋白(vimentin)、肌球蛋白(myosin)、结蛋白(desmin)的变化，与未缺血部分心肌对比，并用计算机图像模拟分析系统计算细胞骨架蛋白量的变化。结果 心肌缺血时30min时即有骨架蛋白actin、myosin的损伤,随后见desmin、vimentin受损。结论 心肌缺血时可以早期导致心肌细胞的细胞骨架损伤。

    【关键词】 心肌缺血；细胞骨架

    The myocytoskeleton damaging in ischemic myocardium

    WANG Meng-jie,ZHANG Yin-huan.Cardiovascular Department of Guangxi People’s Hospital，Nanning 530021,China

    【Abstract】 Objective To study the damaging of several cytoskeleton proteins in ischemic myocardium in animal model.Methods 15 Wistar white mouse,all male,weighing approximately 160g,are selected for study.Ischemia model are made by tie up the left coronary artery,after 30min,60min,120 min,the ischemic tissue is taken out for study,the samples are investigated by immunohistochemistry using monoclonal antibodies against actin ......