黄酮类化合物；_多穗柯；_三波长分光光度法_

三波长分光光度法测定多穗柯叶中黄酮的含量

http://www.100md.com 《时珍国医国药》 2006年第6期

黄酮类化合物；,多穗柯；,三波长分光光度法,,黄酮类化合物；,多穗柯；,三波长分光光度法,摘要,关键词,1方法原理,2实验部分,3结果与讨论,参考文献

     摘要：目的清除由于多穗柯叶中黄酮光谱吸收峰不对称给定量分析造成的影响。方法采用三波长分光光度法测定多穗柯叶中黄酮含量。结果回归方程为△A=0.038 4C+0.001 3；相关系数R=0.999 5；检测波长λ1=460 nm，λ2=420 nm，λ3=380 nm，线性范围为500~3 000 μg/ml，回收率为96.6%~106.2%，变异系数小于0.43%。结论该方法的准确度和精密度均令人满意，且操作简便易行。

    关键词：黄酮类化合物；多穗柯；三波长分光光度法

    Determination of Flavonoids in Lithocarpus Polysachyus rehd Leaves by Three-wavelength spectrophotometry

    XIAO Kunfu

    (College of Lishui,Lishui, Zhejiang 323000, China)

    Abstract：ObjectiveTo solve the infection of asymmetric absorption peak in determination of flavonoids in lithocarpus polysachyus rehd leaves.MethodsDetermination of flavonoids in lithocarpus polysachyus rehd leaves by three-wavelength spectrophotometry.ResultsEquation of linear regression is A=0.038 4C+0.001 3; measuration wavelength is λ1=460 nm, λ2=420 nm, λ3=380 nm ......

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