[摘要]目的: 探讨维生素C对甘露醇引起血管损伤及其诱导的血管内皮细胞凋亡基因表达的影响。 方法: (1)动物实验:将健康大白兔随机分4组，A组在兔耳缘静脉注射甘露醇;B、C、D组分别以生理盐水、维生素C、维生素E预处理后再静脉推注甘露醇，电镜、光镜下观察局部血管的损伤。(2)利用Biostar H-40s型芯片，检测甘露醇分别在应用三种保护剂前后对凋亡基因表达的影响。 结果: (1)动物实验:血管损伤程度上，A组为重度;B组为中～重度;D组为轻度;C组未见明显病理改变。(2)基因芯片检测:330条凋亡基因中，生理盐水组芯片无改变;维生素C组中45条促凋亡基因表达下调，34条抗凋亡基因表达上调;维生素E组3条促凋亡基因表达下调。 结论: 维生素C通过抑制血管内皮细胞凋亡而起到抑制甘露醇血管损伤的作用。

     [关键词] 维生素C;凋亡;血管内皮细胞;甘露醇

    An experiment on inhibitive affection of vitamin C on the apoptosis of vascular endothelial cell induced by mannitol

    PAN Kai-yu，SHANG Shi-qiang，DAI Xiao-na，et al (The People's No.1Hospital of Xiaoshan，Zhejiang Hangzhou 311200，China;Children's Hospital of Zhejiang University，School of Medicine，Zhejiang Hangzhou 310003，China;Shaoyifu Hospital of Zhe-jiang University，School of Medicine，Zhejiang Hangzhou 310003，China)

    [Abstract] Objective:To research the gene expressions of injury on blood vessel and vascular endothelial cell apoptosis induced by mannitol in us-ing vitamin C.Methods:(1)Animal experiment:healthy rabbits were randomized into4groups.Group A was injected with mannitol into the vein of the rabbit ear.Group B ......