胰腺移植·缺血·再灌注损伤·大鼠_

不同热缺血时间再灌注损伤对大鼠移植胰的影响

http://www.100md.com 《中国普通现代外科进展》 2006年第2期

胰腺移植·缺血·再灌注损伤·大鼠,,胰腺移植·缺血·再灌注损伤·大鼠,1材料和方法,2结果,3讨论,参考文献

     【摘要】目的：探讨不同热缺血时间再灌注对大鼠移植胰的损伤情况。方法：6只正常SD大鼠为对照组，18只糖尿病SD大鼠随机分为WIR 0组(热缺血时间0min,n=6)、WIR 15(热缺血时间15min,n=6)和WIR 30(热缺血时间30min,n=6)。WIR 0组、WIR 15组和WIR 30组均行胰腺移植。观测各组再灌注前后血糖，再灌注后2h血清TNFα和NO的含量、移植胰组织中SOD、MPO和MDA含量，组织学变化及细胞凋亡情况。结果：(1)WIR 0组和WIR 15组较再灌注前血糖即下降，再灌注后WIR 15组和WIR 30组较WIR 0组、WIR 30组较WIR 15组血糖高。(2)再灌注后WIR 0组、WIR 15组和WIR 30组较对照组、WIR 15组和WIR 30较WIR 0组、WIR 30较WIR 15组血清TNFα含量高、NO含量低。(3)再灌注后WIR 0组与WIR 15组和WIR 30组较对照组、WIR 15组和WIR 30组较WIR 0组与对照组、WIR 30组较WIR 15组与对照组胰腺组织中SOD活性低、MDA含量高、MPO活性高。(4)再灌注后2hWIR 0组与WIR 15组和WIR 30组较对照组、WIR 15组较WIR 0组凋亡指数高。(5)WIR 30组供胰组织病理损伤最为严重。结论：缺血再灌注损伤可导致移植胰细胞凋亡；在冷缺血180min的条件下，大鼠供胰的最大耐受热缺血时间为15min。

    【关键词】胰腺移植·缺血·再灌注损伤·大鼠

    Effect of the ischemic reperfusion with the different warm ischemic time on pancreas grafts in rats

    LIU Xiaonan1 HUO Tingting2 WANG Weizhong1 GUAN Wenxian1 CHEN Dongli1

    1Department of Gastrointestinal Surgery,2Department of Anaesthesia,Xijing Hospi

    tal,Fourth Military Medical University(Xi’an 710033,China)

    【ABSTRACT】Objective:To investigate the effect of different warm ischemic time on pancreas grafts in rats.Methods:Control group which consist 6 normal SD rats suffer with sham operation,218 steptozozin (STZ)induced diabetic SD rats were randomly assigned to 3 groups:group WIR 0 consisted of 6 diabetic rats which received pancreas transplantation (PT) without warm time ischemic time,Group WIR 15 consisted of 6 diabetic rats which received PT with 15 min ischemic,Group WIR 30 consisted of 6 diabetic rats which received PT with 30 min ischemic time.The blood glucose,NO and TNFα in serum,MDA,SOD,MPO and TUNEL cells in grafts were monitored.Results:(1)The mean blood glucose level of rats post reperfusion in Group WIR 0 and Group WIR 15 was lower than that prereperfusion and that in Group WIR 15 and Group WIR 30 was higher than Group WIR 0 2 hours after reperfusion,WIR 30 was higher than Group WIR 15.(2)The mean TNFα level in serum,MDA and MPO level in grafts in Group WIR 0,WIR 15 and WIR 30 was higher than Group Control 2 hours after reperfusion,and that in Group WIR 15 and WIR 30 was higher than Group WIR 0,and that in Group WIR 30 was higher than Group WIR 15.The mean NO in serum,SOD in grafts level in Group WIR 0,WIR15 and WIR 30 was lower than Group Control2 hours after reperfusion,and that in Group WIR 15 and WIR 30 was lower than Group WIR 0,and that in Group WIR 30 was lower than Group WIR 15.(3)The mean apoptosis index in grafts in Group WIR 0,WIR 15 and WIR 30 was higher than Group Control 2 hours after reperfusion,and that in Group WIR 15 was higher than Group WIR 0.(4)The pathological injury of the grafts in Group WIR 30 were the moist severe.Conclusion:Ischemic reperfusion injury can induce the pancreas graft apoptosis,and with the 180 min cold ischemic time the longest warm ischemic time that the pancreas grafts can endure is 15 min. ......

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