以PHBV为支架构建组织工程化软骨
组织工程;,软骨细胞;,支架;,聚(羟基丁酸酯羟基戊酸酯),,组织工程;,软骨细胞;,支架;,聚(羟基丁酸酯羟基戊酸酯),1材料与方法,2结果,3讨论,参
摘 要:[目的]探讨聚(羟基丁酸酯羟基戊酸酯)(PHBV)多孔材料作为软骨组织工程支架的可行性以及体内外培养方式对软骨形成的影响。[方法]采用“压片-热处理-粒子析出”技术制备PHBV多孔支架。体外分离培养软骨细胞后接种到PHBV支架体外培养2周,期间扫描电镜观察细胞在支架上的生长情况,然后与单纯PHBV支架同植入裸鼠皮下继续培养4、8周后取材,与体外培养至6、10周的细胞-支架复合物同行组织学观察。[结果]电镜观察示软骨细胞在支架上黏附、增殖良好并能分泌细胞外基质;组织学观察示PHBV浅层有新生软骨组织形成,且皮下培养的软骨组织比体外培养的更为成熟。单纯PHBV支架皮下培养没有软骨组织形成。[结论]PHBV可以作为软骨组织工程支架材料,体内培养较体外更有利于组织工程化软骨的形成。关键词:组织工程; 软骨细胞; 支架; 聚(羟基丁酸酯羟基戊酸酯)
Macroporous PHBV matrices for cartilage tissue engineering∥WU Jun,SUN Junying,LI Haiyan,et alDepartment of Orthopaedics,the First Peoples Hospital of Changzhou,Jiangsu 213003,China
Abstract:[Objective]To investigate the possibility of using poly(hydroxybutyratecohydroxyvalerate)(PHBV)as scaffolds for cartilage tissue engineering and to compare the engineered cartilage generated in vitro with those in nude mice modles[Method]PHBV porous scaffolds were fabricated using a compression moulding,thermal processing and salt particulate leaching methodsChondrocytes isolated from articular cartilage were seeded into porous PHBV scaffoldsAfter incubation for 2 weeks in vitro,chondrocytesPHBV constructs were implanted subcutaneously in the dorsum of athymic nude miceControl groups were established by subcutaneous implantation of PHBV aloneThe implants harvested after in vivo incubation of 4 and 8 weeks and cellscaffolds cultured in vitro for 6 and 10 weeks were respectively examined histologicallyChondrocytes cultured for 3,7 and 14 days on the scaffolds were examined by SEM[Result]SEM showed that chondrocytes could aggregate and synthesize extracellular matrix on PHBV scaffoldsBoth specimens harvested from nude mice and those cultured in vitro demonstrated new cartilage formation,while characteristics of the engineered cartilage generated in vivo were more typicalThose from control groups showed no cartilage formation[Conclusion]PHBV can be used as scaffolds for cartilage tissue engineering and nude mice modle seems to facilitate chondrogenesis compared with that in vitro system ......
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