体外冲击波；骨髓间充质干细胞；胰岛素样生长因子Ⅰ；转化生长因

冲击波干预成人骨髓间充质干细胞后IGFⅠ和TGFβ1的表达及意义

http://www.100md.com 《中华首席医学网》 2006年第13期

体外冲击波；骨髓间充质干细胞；胰岛素样生长因子Ⅰ；转化生长因子β1,,体外冲击波；骨髓间充质干细胞；胰岛素样生长因子Ⅰ；转化生长因子β1,冲击波干预成人骨髓间充质干细胞后IGFⅠ和TGFβ1

     摘要：[目的]在体外成功分离培养骨髓间充质干细胞(hMSCs)和测得理想冲击波(ESW)干预能量的基础上，观察体外冲击波干预后成骨活性因子IGFⅠ和TGFβ1表达，探讨其促进成骨作用。[方法]将5kV、100频次的体外冲击波作用于第1代骨髓间充质干细胞，采用免疫细胞化学法隔代观察(P1P11)胰岛素样生长因子Ⅰ(IGFⅠ)和转化生长因子β1(TGFβ1)的表达，计数阳性率；并进行统计学分析，设定P<005为有统计学差异。[结果]ESW干预后的第3代细胞IGFⅠ染色，胞浆及胞核中出现大量黄色棕黄色颗粒，对照组无明显着色；第7代干预组IGFⅠ呈强阳性(879%)；TGFβ1出现较晚，第9代细胞TGFβ1阳性率为722%，随后开始下降；与对照组差异显著(P<001)。[结论]ESW干预能够提高hMSCs增殖活性，IGFⅠ和TGFβ1等的不同时期和不同程度表达是体外冲击波促进hMSCs成骨活动的适时信号和有力证据。

    关键词：体外冲击波；骨髓间充质干细胞；胰岛素样生长因子Ⅰ；转化生长因子β1

    Expression and signififcance of IGFⅠ TGFβ1 in human mesenchymal stem cells after exposured to extracoporeal shock wave∥WANG Wuzhou,XING Gengyan,YE Qibin,et alDepartment of Orthopedics,General Hospital of the Chinese Peoples Armed Police Forces,Beijing 100039，China

    Abstract：[Objective]To demonstrate mechanism of ESW in curing osteogetic disorders,we studied expression of some osteogenetic factors in human mesenchymal stem cells(hMSCs)when exposed to ESW[Method]After success in marrow aspiration,isolation and obtainment optimal experimental energy,a dose of 5kV and 100 times of ESW was applied to hMSCs of passage 1The expression of IGFⅠ and TGFβ1 were examined by immunocytochemical staining[Result]The cytochemical staining results showed that expression of IGFⅠ and TGFβ1 appeared at different passage of hMSCs after ESW interventionAppearance of IGFⅠ was earlier than TGFβ1 which didnt express until passage 7At the same interval,the expression of IGFⅠ and TGFβ1 in control group difference is lower than ESW group,respectively(P<001)[Conclusion]ESW of 5kV and 100 times can improve hMSCs proliferation,which is associated with the appearance of osteogenetic factors,such as IGFⅠ and TGFβ1 ......

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