川芎嗪对AngⅡ诱导的VSMCs增殖中CaM和CaN活性的影响
川芎嗪；,VSMCs；,钙调蛋白；,钙调神经磷酸酶,,川芎嗪；,VSMCs；,钙调蛋白；,钙调神经磷酸酶,1材料与方法,2结果,3
摘要：目的 探讨不同浓度川芎嗪在不同作用时间内对血管紧张素Ⅱ(AngⅡ)诱导的VSMCs(VSMCs)增殖的抑制作用。方法 建立AngⅡ诱导VSMCs增殖模型，用酶促反应定磷法观察不同浓度的川芎嗪在不同时段内对VSMCs中钙调蛋白(CaM)和钙调神经磷酸酶(CaN)活性影响。结果 成功建立AngⅡ诱导的VSMCs增殖模型，川芎嗪各组CaM和CaN活性随川芎嗪的浓度和作用时间的延长而显著下降(P<0.01)。结论 川芎嗪对AngⅡ诱导的VSMCs增殖的抑制作用有剂量和时间依赖性，其抑制机制可能与其干预CaN依赖的信号转导途径有关。关键词：川芎嗪； VSMCs； 钙调蛋白； 钙调神经磷酸酶
Effects of Tetramethylpyrazine on the
Proliferation of Vascular Smooth Muscle Cells
WANG Meijuan ,ZHENG Honghua,LUO Desheng，et al
(Department of Biochemistry,Xianning College，Hubei Xianning 437100 China)
ABSTRACT:Objective To study the effects of tetramethylpyrazine(TMP) on calmodulin(CaM) and calcinuerin(CaN) in the proliferation of vascular smooth muscle cells(VSMCs) induced by AngⅡ.Methods A cells proliferating model of VSMCs induced by angiotensinII(AngⅡ) was established; the effects of tetramethylpyrazine(TMP) on CaM and CaN were detected by enzyme reaction phosphorus measurement.Results Cell count,cell proliferation activity, CaM and CaN activities in VSMCs were obviously reduced as compared with Ang II group in a dose and timedependent manner while treated with TMP(P<0.01). Conclusion The VSMCs proliferation induced by AngII can be inhibited by TMP in a dose and timedependent manner and the inhibiting mechanism may be related to inhibiting CaM and CaN activities. ......
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