人大肠癌HCT-8/5-FU耐药细胞株的建立及P-gp测定
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布立民, 孙淑红, 华建平, 韩 英, 赖 靖,
肿瘤细胞;细胞培养;大肠癌;耐药细胞株;多药耐药;P-糖蛋白,布立民,华建平,鲍文漪,孙淑红,韩英,赖靖,布立民,通讯作者,Preparat
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布立民, 华建平, 鲍文漪, 天津市第一中心医院消化科 天津市 300000
孙淑红, 承德医学院外科教研室 河北省承德市 063700
韩英, 北京军区总医院消化科 北京市 100700
赖靖, 解放军464医院内二科 天津市 300810
布立民, 2003年军医进修学院硕士生, 主治医师, 主要从事胃肠道肿瘤的诊治研究.
通讯作者: 韩英, 100700, 北京市东城区南门仓5号, 北京军区总医院消化内科.
电话: 010-66721009
收稿日期: 2006-05-16 接受日期: 2006-06-05
Preparation of HCT-8/5-fluorouracil multidrug resistant cell line and detection of P-glycoprotein
Li-Min Bu, Shu-Hong Sun, Jian-Ping Hua, Ying Han, Jing Lai, Wen-Yi Bao
Li-Min Bu, Jian-Ping Hua, Wen-Yi Bao,Department of Gastroenterology, the First Central Hospital, Tianjin 300000, China
Shu-Hong Sun,Department of Surgery, Chengde Medical College, Chengde 063700, Hebei Province, China
Ying Han,Department of Gastroenterology, General Hospital of Beijing Military Command, Beijing 100700, China
Jing Lai,the Second Department of Internal Medicine, the 464th Hospital of Chinese PLA, Tianjin 300810, China
Correspondence to:Dr. Ying Han, Department of Gastroenterology, General Hospital of Beijing Military Command, 5 Nanmencang Road, Dongcheng District, Beijing 100700, China.
Received:2006-05-16 Accepted:2006-06-05
Abstract
AIM: To develop HCT-8/5-fluorouracil multidrug resistant cell line and explore the mechanism its drug resistance.
METHODS: High concentration of 5-FU was used in the beginning to induce drug resistance of HCT-8 cell line, and thereafter, the concentration of 5-FU was increased in gradient. About 7 mo later, the cells could stably grow in 2.0 mg/L 5-FU, which was named HCT-8/5-FU multidrug resistant cell line. The resistant index of HCT-8/5-FU cells to 5-FU, adriamycin (ADM), and cisplatin (DDP) was examined with MTT assay, and growth curve was drawn. The morphological changes were observed by both light and electron microscopy. The function of P-glycoprotein (P-gp) was detected by flow cytometry using rhodamine staining ......
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