肝炎病毒；乙型；HBVDNA；多态性_____]

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乙型肝炎患者HBVDNA与HBVM及HBVDNA前C/C区变异的对比研究

http://www.100md.com 《实用医技杂志》 2006年第10期

肝炎病毒；乙型；HBVDNA；多态性,,],肝炎病毒；乙型；HBVDNA；多态性,1材料与方法,2结果,3讨论

     [摘要] 目的：探讨乙型肝炎患者的乙型肝炎病毒血清学标志(HBVM)与HBVDNA及HBVDNA前C/C区变异检测结果的相关性与临床意义。方法：对359例乙型肝炎患者血清的HBVM和HBVDNA及HBVDNA前C区1 896/1 814位变异检测结果进行比较；HBVM用ELISA定性分析法检测；HBVDNA用PCR荧光定量法检测；HBVDNA前C/C区用基因芯片显色法检测。结果：急性乙型肝炎、慢性乙型肝炎、乙型肝炎肝硬化HBVDNA检出率分别为81.8%、62.4%、44.7%，三组进行组间比较，差异均有显著性(P<0.01)；HBsAg与HBeAg均阳性组HBVDNA 检出率显著高于HBsAg与抗HBe均阳性组(P<0.01)；HBsAg与抗HBe均阳性组的变异率高达50.7%，显著高于HBsAg与HBeAg均阳性组(P<0.01)。结论：HBVDNA是评价HBV活动最理想的标志；HBVDNA前C/C区变异在我国乙型肝炎患者中普遍存在，对抗HBe阳性患者的临床意义更大；抗HBe的出现不能作为HBV复制停止的指标；HBVDNA量的变化可作为临床评价病毒复制和抗病毒疗效的参考指标。

    [关键词] 肝炎病毒；乙型；HBVDNA；多态性

    Study of Difference among HBVDNA,HBVM and Polymorphism of HBVDNA prec/cregion in Cases with Hepatitis B

    SHAN Chunmei，WANG Wanxiang (Yancheng First People's Hospital,Yancheng,Jiangsu 224006,China)

    Abstract：Objective Serum samples of 359 cases with hepatitis B were detected for hepatitis B virus markers(HBVM),HBVDNA and polymorphism of HBVDNA prec/cregion,in order to investigation the relationship among HBVDNA,HBV M and polymorphism of HBVDNA prec/cregion,and its clinical significant.Methods HBVM were detected by ELISA.HBVDNA were detected by PCR.Polymorphism of HBVDNA prec/cregion were detected by HBV gene chip.Results HBVDNA positive rates about acute,chronic hepatitis B and liver cirrhosis were 81.8%,62.4% and 44.7%.The positive rates of HBVDNA in the three groups showed significant differences(P<0.01).HBVDNA positive rate in the HBsAg and HBeAg positive group was higher than that in the HBsAg and antiHBe positive group(P<0.01).The HBVDNA of HBsAg and antiHBe positive group mutation rate was 50.7%,which was higher than that in the HBsAg and HBeAg positive group(P<0.01).Conclusion HBVDNA can be treated as a sign of HBV activity.The polymorphism of HBVDNA prec/cregion commonly existed in chronic hepatitis B patients of the our country,this study has bigger clinical significance in hepatitis B patients of antiHBe.The appearance of antiHBe can not be taken as indicator of ceasing of HBV replication.The changes in HBVDNA titers may be used as clinical evaluation on HBV replication and index for antiviral therapeutic effect. ......

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