【摘要】 目的 建立用内标法测定人血浆中头孢羟氨苄浓度的HPLC-UV方法。方法 用10%高氯酸直接沉淀血浆蛋白，以对氨基苯甲酸为内标，测定血浆中头孢羟氨苄的浓度。结果 血浆中杂质不干扰样品峰和内标峰，20.0、2.0、0.2μg/ml的回收率分别为98.1%，98.7%， 91.1%；批内精密度均小于8.0%(高、中、低浓度分别为3.8%，5.6%，7.3%)，批间精密度均小于10%(高、中、低浓度分别为 4.1%，6.5%，9.7%)，最低检测限为0.1μg/ml，线性范围为0.2～30.00μg/ml；血浆中头孢羟氨苄冻融是稳定的；精密度也符合要求。结论 该方法稳定且精密度好，准确可靠，适用于头孢羟氨苄血浆浓度测定。

    【关键词】 头孢羟氨苄；对氨基苯甲酸；高效液相色谱-紫外检测器

    【Abstract】 Objective To establish the determination method of cefadroxil in healthy volunteers plasma. Methods Plasma protein was precipitated by perchloric acid (10%), and paraaminobenzoic acid was used as internal standard. Plasma concentrations of cefadroxil and internal standard were determined by HPLC-UV.Results The chromatographic peak of cefadroxil and internal standard was not interfered by foreign matter. Recovery rate of 20.0、2.0、0.2μg/ml was 98.1%, 98.7%, 91.1%, respectively. Precision in or between batch was less than 8.0% and 10%, respectively. Lowest detectable limit was 0.1μg/ml and linear range was 0.2～30.00μg/ml. The freeze thawing of plasma was stable.Conclusion The method was reliable and stable, it was suitable for determination the concentration of cefadroxil in plasma. ......