【摘要】 目的 构建膀胱移行癌7q31.1区域内的杂合性缺失精细图谱，为进一步寻找膀胱移行癌相关基因提供依据。方法 采用显微切割的方法获取较纯的肿瘤组织，选用7个微卫星标志结合PCR技术对 30例膀胱移行癌进行杂合性缺失分析。结果 30例膀胱移行癌组织中，至少有一个位点出现杂合性缺失者 21例，占70%(21/30)；D7S2459、D7S523、D7S2502、D7S486、D7S480、D7S650、D7S2486各位点杂合性缺失频率分别为10.0%、6.7%、23.3%、43.3%、26.7%、26.7%、20.0%；缺失图谱分析显示部分膀胱移行癌常见最小缺失区域位于D7S2502-D7S480之间。结论 膀胱移行癌染色体7q31.1区域发生高频率杂合性缺失，提示该区域可能存在与膀胱移行癌发生有关的抑癌基因。

    【关键词】 膀胱移行癌；染色体7q31.1区域；抑癌基因

    Study of loss of heterozygosity at chromosome 7q31.1 in transitional cell carcinoma of urinary bladder

    ZHANG Feng-hua，LIAO Kun，SONG Xing-zhi.Clinical Medical School，Xiangna College，Chenzhou 423000，China

    【Abstract】 Objective To construct a detailed mapping of the chromosome 7Q31.1 deleted region in transitional cell carcinoma(TCC)by polymerase chain reaction-loss of heterozygosity (PCR-LOH)analysis for the further research of TCC-related gene(s).Methods Relatively pure tumor tissues were obtained by microdissection.PCR was used to perform LOH analysis of TCC samples using D7S2459 ......