【摘要】 目的 体外研究化疗药物顺铂诱导胰腺癌细胞株(Panc-1)胞内凋亡抑制蛋白Survivin的表达变化，探讨Survivin在胰腺癌化疗抵抗中的作用机制。方法 用人胰腺癌细胞株Panc-1株进行培养传代；MTT实验检测顺铂以不同浓度和不同时间对Panc-1株生长的抑制作用；用RT-PCR检测凋亡过程中Survivin基因表达的动态变化。结果 顺铂可明显抑制Panc-1细胞增殖，其增殖抑制率呈浓度和时间依赖性；DNA电泳可以观察到凋亡的发生，而且呈药物浓度、作用时间依赖关系；Survivin表达水平随着顺铂作用浓度的增加而下降，当顺铂浓度为50μg/ml时下降最明显(61%)；Survivin cDNA/β-actin cDNA比值与细胞增殖抑制率和细胞凋亡率呈负相关(P<0.01)。结论 顺铂可以有效诱导Panc-1株的细胞凋亡，而且Survivin基因的抑制在顺铂诱导Panc-1株的细胞凋亡中起重要作用。

    【关键词】 Survivin； 顺铂；胰腺癌； 凋亡

    Apoptosis protein of survivin expression induced by cisplatin in cell of pancreatic cancer

    ZOU Jie,GUO Kai-wen,TANG Chao-hui.

    Department of Oncology,the Second Hospital of the Wuhan Steel Company,Hubei 460085,China

    【Abstract】 Objective To study the expression of survivin induced by cisplatin in Panc-1 cells in vitro, so as to provide the role of survivin on the mechanisms of chemotherapy drug resistance. Methods The pancreatic cancer cell line Panc-1 was chosen in this experiment. The inhibitory effects of chemotherapeutical drugs on Panc-1 cell line were assayed with MTT test. The cisplatin group were divided into different groups by concentration and time. Expression of survivin detected by RT-PCR. Results Cisplatin obviously inhibited the Panc-1 cell growth with dose- and time-dependent. Occurrence of apoptosis was detected by DNA electrophoresis staining in a dose- and time-dependent manner. Survivin expression was decreased with increasing of cisplatin concentration,its maximum effect was achieved at a concentration of 50μg/ml,at which mRNA was down-regulated by 61%.The ratio of survivin and β-actin was negatively with the inhibitory rate of cell proliferation and the rate of apoptosis, P<0.01.Conclusion Cisplatin can effectively induce apoptosis in Panc-1 cells and the inhibition of survivin gene expression may play a critical role on the Panc-1 cell apoptosis induced by cisplatin. ......