亚细胞毒性剂量As2O3增加胃癌细胞对rhTRAIL的敏感性及其机制
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仲 飞, 朱兆华, 张世能
肿瘤坏死因子相关的凋亡诱导配体;三氧化二砷;胃癌;凋亡,亚细胞毒性剂量As2O3增加胃癌细胞对rhTRAIL的敏感性及其机制,仲飞,朱兆华,张世能,仲飞,通讯作者:,Subcytotoxicc
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仲飞, 朱兆华, 张世能, 中山大学附属第二医院消化科 广东省广州市 510120
仲飞, 中山大学2004级在读博士, 主要从事消化系统肿瘤的基础与临床研究.
通讯作者: 朱兆华, 510120, 广东省广州市沿江西路107号, 中山大学附属第二医院消化科. zhuzhaohua@163.com
电话: 020-81332490
收稿日期: 2006-06-28 接受日期: 2006-08-10
Subcytotoxic concentration of arsenic trioxide enhances the sensitivity of SGC7901 cells to rhTRAIL and its mechanism
Fei Zhong, Zhao-Hua Zhu, Shi-Neng Zhang
Fei Zhong, Zhao-Hua Zhu, Shi-Neng Zhang,Department of Gastroenterology, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China
Correspondence to:Zhao-Hua Zhu, Department of Gastroenterology, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China. zhuzhaohua@163.com
Received:2006-06-28 Accepted:2006-08-10
Abstract
AIM: To investigate the effects of subcytotoxic concentration of arsenic trioxide (As2O3) on the rhTRAIL-induced apoptosis in SGC7901 cells and its related mechanism.
METHODS: The human gastric cancer cell line SGC7901 was treated with As2O3 (1 μmol/L), rhTRAIL (500 μg/L), respectively, or in combination. The cell apoptosis was detected by flow cytometry (AnnexinV-FITC-PI assay); the expression of TRAIL R1/DR4 and TRAIL R2/DR5 on the cell surface were determined by indirect fluorescence staining and flow cytometry; the expression of TRAIL R1/DR4 and TRAIL R2/DR5 mRNA was determined by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: Used along or in combination with As2O3, rhTRAIL induced apoptosis of SGC7901 cells in a time-dependent manner (12-72 h). After being exposed to rhTRAIL in combination with As2O3 for 24, 48 and 72 h, the apoptosis rates of SGC7901 cells were significantly higher than those of cells treated with the same concentration of rhTRAIL alone (36 ......
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