多孔整体固定相的制备与色谱性能
整体柱,甲基丙烯酸缩水甘油酯二甲基丙烯酸乙二酯,温度控制,孔结构,径向流动,,整体柱，甲基丙烯酸缩水甘油酯二甲基丙烯酸乙二酯，温度控制，孔结构，径向流动,1引
摘 要 结合连续加料降低聚合体系的放热量，及强化体系的换热能力等措施，实现了无搅拌模式聚合体系中温度分布的均一化。通过单次聚合过程构造了体积为38 mL的大体积整体固定相。SEM及孔隙率分布测试证明了整体固定相具有均匀的孔结构，动态容量测定及分离蛋白质的实验，表明所制备的径向流动模式整体柱的容量及分辨率不依赖于流速，同时在高流速下具有低背压的特征，可用于生物大分子的分离制备。关键词 整体柱，甲基丙烯酸缩水甘油酯二甲基丙烯酸乙二酯，温度控制，孔结构，径向流动
Preparation Porous Monolithic Column and
Its Chromatographic Performance
Chen Guoliang, Zhang Xiaohui, Shang Defa, Wang Qingbin, Yang Changlong, Li Tong
1(School of Chemical Engineering of Qiqihar University, Qiqihar 161006)
2(Dalian Elite Analytical Instruments CO., Ltd., Dalian 116023)
Abstract A 38mL monolith with homogeneous porous structure was produced by a single polymerization from glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) in the presence of porogens and an initiator. The uniform temperature distribution within the reaction system was achieved by adding reactant mixture continuously and enhancing the heat transfer ability of the polymerization system. Homogeneous porous structure in the monolith was proved by SEM and the pore size distribution profiles measured by mercury intrusion porosimetry. Experimental results from proteins separation indicated that the dynamic capacity and resolution of radial flow monolithic column were independent of flow rates. Furthermore, the pressure drop on the column was linearly dependent on the flow rate and did not exceed 1.7 MPa even at a flow rate of 50 mL/min, which proved that the prepared monolith can be used in the quick separation and preparation of biopolymers. ......
您现在查看是摘要页，全文长 11293 字符。