肝癌细胞系放射诱导的细胞凋亡和细胞周期的变化
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董广璐, 邢丽娜, 刘晓滨, 刘 伟, 金 茜,
X射线;细胞周期;细胞凋亡;细胞系;流式细胞术,董广璐,邢丽娜,刘晓滨,刘伟,金茜,张淑云,董广璐,通讯作者:,Propertiesofra
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董广璐, 邢丽娜, 刘晓滨, 哈尔滨医科大学附属第二医院肿瘤放射治疗科 黑龙江省哈尔滨市 150086
刘伟, 金茜, 张淑云, 哈尔滨医科大学附属第二医院科研实验中心 黑龙江省哈尔滨市 150086
董广璐, 1993年哈尔滨医科大学硕士, 副主任医师, 副教授. 主要从事肿瘤放射治疗.
黑龙江省科技计划攻关项目, No. GC03C606-4
通讯作者: 张淑云, 150086, 哈尔滨市南岗区学府路246号, 哈尔滨医科大学附属第二医院科研实验中心.
zhshuyun136@yahoo.com.cn 电话: 0451-86605487 传真：0451-86688011
收稿日期: 2006-08-29 接受日期: 2006-09-28
Properties of radiation-induced apoptosis and cell cycle changes in liver cancer cell lines
Guang-Lu Dong, Li-Na Xing, Xiao-Bin Liu, Wei Liu, Qian Jin, Shu-Yun Zhang
Guang-Lu Dong, Li-Na Xing, Xiao-Bin Liu,Department of Tumor Radiotherapy, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Wei Liu, Qian Jin, Shu-Yun Zhang,Research Center, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Supported bythe Key Science and Technology Project of Heilongjiang Province, No. GC03C606-4
Correspondence to:Shu-Yun Zhang, Research Center, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China. zhshuyun136@yahoo.com.cn
Received:2006-08-29 Accepted:2006-09-28
Abstract
AIM: To analyze the properties of radiation-induced apoptosis and cell cycle changes in liver cancer cell lines.
METHODS: Liver cancer cell lines HepG2 and SMMC-7721 were included in this study, and normal liver cell line HL-7702, pulmonary small cell carcinoma cell line HCI-H460 and lung adenocarcinoma cell line A549 were used as controls. After routine culture for 48 h, all the cells were irradiated under 4Gy X-radiation. The cell cycle and apoptosis were detected by flow cytometry (FCM) before and 6, 12, 24, 36, 48 h after irradiation.
RESULTS: After irradiation, cell apoptosis started from the 6th h and reached to the peak at 12 h in HepG2 cells (ΔApoHepG2 = 45 ......
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