蛋白质二硫键异构酶在幽门螺杆菌感染者胃黏膜中的表达及意义
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王孟春, 郭静波, 刘明华, 方文刚, 李 岩
幽门螺杆菌;蛋白质二硫键异构酶,王孟春,郭静波,刘明华,李岩,方文刚,通讯作者:,Expressionofproteindisulfi
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王孟春, 郭静波, 刘明华, 李岩, 中国医科大学附属第二医院消化内科 辽宁省沈阳市 110004
方文刚, 中国医科大学发育生物教研室 辽宁省沈阳市 110001
辽宁省教育厅基金资助项目, No. 202010794
通讯作者: 王孟春, 110004, 辽宁省沈阳市, 中国医科大学附属第二医院消化内科. wangmc@cmu2h.com
电话: 024-83956416 传真: 024-23892617
收稿日期: 2006-06-20 接受日期: 2006-08-14
Expression of protein disulfide isomerase in gastric mucosa infected with Helicobacter pylori
Meng-Chun Wang, Jing-Bo Guo, Ming-Hua Liu, Wen-Gang Fang, Yan Li
Meng-Chun Wang, Jing-Bo Guo, Ming-Hua Liu, Yan Li,Department of Gastroenterology, the Second Affiliated Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
Wen-Gang Fang,Department of Developmental Biology, College of Basic Medical Science, China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to:Meng-Chun Wang, Department of Gastroenterology, the Second Affiliated Hospital of China Medical University, Shenyang 110004, Liaoning Province, China. wangmc@cmu2h.com
Received:2006-06-20 Accepted:2006-08-14
Abstract
AIM: To study the expression of protein disulfide isomerase (PDI) in gastric mucosa infected with H pylori.
METHODS: Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to examine the mRNA and protein expression of PDI, respectively, in human gastric mucosa with or without H pylori infection (n = 32 or 28).
RESULTS: The expression amounts of PDI mRNA and protein were 0.5704 ± 0.0794 and 0.5198 ± 0.0379 in the mucosa without H pylori infection, which were significantly lower than those in the ones with H pylori infection (1.0642 ± 0.1533, 0.8252 ± 0.0321, P < 0.01).
CONCLUSION: H pylori infection can increase the transcription of PDI mRNA and synthesis of PDI protein in gastric mucosa ......
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