四株人结肠腺癌细胞CEA表达水平的检测及其意义
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王立峰, 刘宝瑞, 钱晓萍, 殷海涛, 禹立霞,
癌胚抗原;人结肠癌细胞;半定量RT-PCR;免疫组化王立峰,刘宝瑞,钱晓萍,殷海涛,禹立霞,魏嘉.四株人结肠腺癌细胞CEA表达水平的检测及其意义. 世界华人消化杂志2006;14(30)2
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王立峰, 刘宝瑞, 钱晓萍, 殷海涛, 禹立霞, 魏嘉, 南京大学医学院附属鼓楼医院肿瘤中心 南京大学临床肿瘤研究所 江苏省南京市 210008
王立峰, 南京大学医学博士, 南京大学医学院附属鼓楼医院肿瘤中心主治医师, 主要从事恶性肿瘤的分子生物学研究.
通讯作者: 刘宝瑞, 210008, 江苏省南京市, 南京大学医学院附属鼓楼医院肿瘤中心\南京大学临床肿瘤研究所.
baoruiliu@nju.edu.cn
电话: 025-83304616-21002
收稿日期: 2006-08-16 接受日期: 2006-09-16
Expression of carcinoembryonic antigen in four different human colon adenocarcinoma cell lines
Li-Feng Wang, Bao-Rui Liu, Xiao-Ping Qian, Hai-Tao Yin, Li-Xia Yu, Jia Wei
Li-Feng Wang, Bao-Rui Liu, Xiao-Ping Qian, Hai-Tao Yin, Li-Xia Yu, Jia Wei, Oncology Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School; Clinical Oncology Institute of Nanjing University, Nanjing 210008, Jiangsu Province, China
Correspondence to: Bao-Rui Liu, Oncology Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School; Clinical Oncology Institute of Nanjing University, Nanjing 210008, Jiangsu Province,China. baoruiliu@nju.edu.cn
Received: 2006-08-16 Accepted: 2006-09-16
Abstract
AIM:To investigate the expression of carcinoembryonic antigen (CEA) and its significance in the four different colon adenocarcinoma cell lines.
METHODS: Enzyme-linked immunosorbent assay (ELISA) was used to measure the expression of CEA in the cell culture medium and the immunohistochemistry was used to detect the protein expression of CEA in LS174T, SW480, LoVo and HCT-8 cell lines, separately. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) for the expression of CEA mRNA in the four kinds of cell lines was also carried out.
RESULTS: A great variation in CEA content was found among the four kinds of colon cancer cell lines. The expression of CEA was detectable at the protein and mRNA level in LS174T and SW480 cell lines, and moreover, it was higher in the former (culture supernatant fluid: 1050 ± 25.0 ng/107 cells vs 66 ± 5 ......
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