Effects of 17ª²¦Â estradiol on apoptosis and mRNA expression of MT1ª²MMP in endothelial cell under high glucose condition

    WANG Lei, LIU Jing, XIA Guangª²Hao

    Clinical Medical School, Lanzhou University, Department of Endocrinology, People¬ðs Hospital of Gansu Province, Lanzhou 730000, China

    ¡¾Abstract¡¿ AIM: To explore the effect of 17ª²¦Â estradiol (E2)on apoptosis and membraneª²type matrix metalloproteinase 1(MT1ª²MMP) mRNA expression in endothelial cell line EVCª²304 under the high glucose condition 30 mmol/L. METHODS: The proliferation of EVCª²304, which is treated by high glucose(30 mmol/L) and/or E2 of different concentrations respectively, was measured by MTT assays. Then the apoptosis of ECVª²304 was detected by flow cytometry(FCM) and DNA ladder assays. Finally, MT1ª²MMP mRNA expression was measured by semiª²quantitative RTª²PCR. RESULTS: After ECVª²304 had been treated by high glucose(30 mmol/L) ,its proliferation and mRNA expression of MT1ª²MMP was inhibited significantly(P<0.01). However, the tendency fall back after E2 had been administrated(P<0.01). Furthermore, this effect of E2 reached a peak at its concentration from 0.01 to 0.10 mmol/L. The apoptosis was only detected in cells which had been administrated by high glucose and E2 at low (0.001 nmol/L) and high (1.0 nmol/L) concentration by DNA Ladder assays. CONCLUSION: High glucose has a strong toxicity on vascular endothelial cells. Moderate level of E2 has a protective effect on vascular endothelial cells. E2 can enhance MT1ª²MMP expression of endothelial cells under high glucose condition. ......