Treatment of rat cranial defect with autogenetic minimal morselized bone and allogenic cells

    MA Song, YAN Jingª²Long, LIN Xin, PAN Haiª²Tao, WANG Xinª²Tao, YANG Xianª²Sheng

    Department of Orthopaedics, Tiantan Hospital, Capital Medical University,Beijing 100050, China, Department of Orthopaedics, First Affiliated Hospital, Harbin Medical University, Harbin 150001, China

    ¡¾Abstract¡¿ AIM: To investigate the combined treatment effects of autogenetic minimal morselized bone and allogenic cells on rat cranial defect. METHODS: Cranial defect in rat models was repaired with autogenetic minimal morselized bone graft and allogenic cell transplantation[osteoblasts or marrow stormal cells (MSC)]. X ray was taken to understand the union condition, and RTª²PCR was performed to examine the mRNA expressions of TGFª²¦Â1 and bFGF. RESULTS: In the order of combined treatment group A (morselized bone and osteoblast graft), combined treatment group B(morselized bone and MSC graft), combined treatment group C(minimal morselized bone only), the union time of cranial defect prolonged as (7.33¡À0.44) wk, (8.67¡À0.69) wk and (11.67¡À1.52) wk, and the expression of TGFª²¦Â1 and bFGF emerged in the order (P<0.05 between every 2 groups). CONCLUSION: The combined therapy of minimal morselized bone and allogenic cells accelerate the repairment of cranial defect and the expression of cell factors. ......