Influence of adiponectin on vascular inflammatory reaction mediated by TNFª²¦Á

    LIU Jinª²Bo, DENG Huaª²Cong, GE Qian, LI Bingª²Rong, ZHENG Hongª²Ting, LAN Liª²Zhen

    Department of Endocrinology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China

    ¡¾Abstract¡¿ AIM: To investigate the influence of adiponectin on the inflammatory reaction in vascular endothelial cells induced by TNFª²¦Á. METHODS: Human umbilical vein endothelial cells(HUVEC) were cultured in vitro and divided into normal group, TNFª²¦Á stimulation group and adiponectin and TNFª²¦Á associated stimulation group. HUVEC were preincubated with human recombinant adiponectin for 30 min to 16 h, then exposed to human recombinant TNFª²¦Á for 6 to 12 h (adiponectin and TNFª²¦Á associated stimulation group), which was then compared with TNFª²¦Á stimulation group in the expressions of NO, iNOS, ETª²1 and MCPª²1. The interaction between adiponectin and HUVEC was estimated by chemical method, radioimmunoassay and ELISA. RESULTS: The expressions of NO, iNOS, ETª²1 and MCPª²1 were increased in HUVEC stimulated by TNFª²¦Á for 6 to 12 h (607.7¡À7.6, 42.2¡À2.2, 199.3¡À11.9, 167.7¡À15.9 vs 543.5¡À2.2, 23.8¡À2.0, 148.4¡À5.3, 128.0¡À8.4; P<0.05). Compared with TNFª²¦Á stimulation group, the expressions of NO, iNOS, ETª²1 and MCPª²1 in adiponectin and TNFª²¦Á associated stimulation group were decreased (565.7¡À13.0, 36.5¡À2.7, 170.8¡À8.3, 142.6¡À5.6 vs 607.7¡À7.6, 42.2¡À2.2, 199.3¡À11.9, 167.7¡À15.9; P<0.05). CONCLUSION: Adiponectin can modulate endothelial inflammatory response through effecting the expressions of some inflammatory factors, and adiponectin has protective actions against the initiation and progression of atherosclerosis. ......