Change of Na+, K+ª²ATPase ¦Á isoforms in rat neurons damaged by global cerebral ischemia/reperfusion

    GUO Fang, L¦a Ping, LI Shuª²Min, WANG Yongª²Li

    Department of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China

    ¡¾Abstract¡¿ AIM: To investigate the change of Na+, K+ª²ATPase ¦Á isoforms in rat neurons damaged by global cerebral ischemia/reperfusion. METHODS: The globral cerebral ischemia (15 min) in rats were induced by Pulsinelli 4ª²VO method and the rats were reperfused for 30 min. With RTª²PCR and immunohistochemical assay, the expression of the Na+, K+ª²ATPase ¦Á isoforms was detected in rat hippocampal and cortical neurons following ischemia/reperfusion. RESULTS: The pyramidal cells during ischemia/reperfusion displayed notable edema. The ¦Á1, ¦Á2 or ¦Á3 isoform of Na+, K+ª²ATPase distributed in a tissueª²specific fashion in neurons of hippocampus and cortex, and ¦Á3 isoform was more than ¦Á2 and ¦Á1 isoforms in hippocampus, while there was no significant difference in cortex. The expressions of ¦Á1 and ¦Á3 isoforms in the rats suffering ischemia/reperfusion were significantly decreased in hippocampus (P<0.05 or 0.01). However, the expressions of ¦Á1 and ¦Á3 in cortex did not change during 15ª²min ischemia and declined in 30ª²min reperfusion (P<0.05). In both ischemia and reperfusion, the mRNA expressions of ¦Á1 and ¦Á3 isoforms at mRNA level had no significant change as compared with shamª²operated group. CONCLUSION: The change of the expressions of ¦Á1 and ¦Á3 isoforms may involve in global cerebral ischemia/reperfusioninjury. ......