KOH涂片_____角膜炎；真菌；培养

KOH涂片镜检法诊断真菌性角膜炎的意义

http://www.100md.com 《广东药学院学报》 2006年第2期

KOH涂片,,角膜炎；真菌；培养,1资料与方法,2结果,3讨论,参考文献:

     【摘要】目的:探讨KOH涂片镜检法对真菌性角膜炎的诊断意义，了解湛江地区真菌性角膜炎的病原学。方法:取102例真菌性角膜炎患者的角膜溃疡标本，作KOH涂片镜检、真菌培养及菌种鉴定。结果:KOH涂片镜检法和真菌培养法的阳性率分别为97.1%(99/102)、94.9%(93/98)，二者的真菌检出率差异无显著性(P>0.05)。93份培养阳性标本中，共分离出18属33种真菌，其中镰刀菌属、弯孢霉属和曲霉属真菌分别占36.6%、27.9%、7.5%。结论:KOH涂片镜检法是早期诊断本病的简单、有效方法。镰刀菌和弯孢霉是湛江地区真菌性角膜炎的主要致病菌。

    【关键词】角膜炎；真菌；培养

    Diagnostic value of potassium hydroxide based smear assay for fungal keratitis

    YANG Liping1, HUANG Wenming2, FAN Yiming2, LI Ruizhuang3

    (1. Laboratory Department, The Affiliated Hospital of Guangdong Medical College,

    Zhanjiang 524001, China)

    【Abstract】 Objective: To investigate the diagnostic value of potassium hydroxide based smear assay for mycotic keratitis and the pathogenic spectrum in Zhanjiang area. Methods: The corneal scrapings from 102 patients with fungal keratitis were subjected to 10% KOH based smear before microscopic observation, fungal culture and strain identification. Results: The positive rates of fungal infection of fungal keratitis detected by KOH based smear and fungal culture were 97.1% (99/102) and 94.9% (93/98), respectively, and there was no significant difference in the fungal detection efficiency between the two methods (P>0.05). The pathogenic fungi belonged to 18 genus and 33 species； of which, Fusarium, Curvularia and Aspergillus accounted for 36.6%, 27.9% and 7.5%, respectively. Conclusion: The KOH based smear is simple and effective in the early diagnosis of fungal keratitis. Fusarium and Curvularia are the predominant pathogens of mycotic keratitis in Zhanjiang district. ......

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