转染VEGF165的大鼠血管内皮细胞与胰岛共移植对糖尿病大鼠的治疗作用
http://www.100md.com
程 颖, 刘永锋, 张佳林, 李铁民, 赵 宁
胰岛移植;再血管化;血管内皮生长因子165;基因转染;血管内皮细胞,转染VEGF165的大鼠血管内皮细胞与胰岛共移植对糖尿病大鼠的治疗作用,程颖,刘永锋,张佳林,李铁民,赵宁,程颖,通讯作者:
 |
| 第6页 |
 |
| 第1页 |
参见附件(1210KB，7页)。
程颖, 刘永锋, 张佳林, 李铁民, 赵宁, 中国医科大学附属第一医院器官移植科 辽宁省沈阳市 110001
程颖, 2005年中国医科大学博士, 助理研究员, 主要从事胰岛移植、器官保存方面的研究.
高等学校博士学科点专项科研基金资助项目, No. 20050159010
通讯作者: 程颖, 110001, 辽宁省沈阳市和平区南京北街155号, 中国医科大学附属第一医院器官移植科. chengying75@sina.com
电话: 024-83282452
收稿日期: 2006-07-03 接受日期: 2006-09-01
Co-transplantation of VEGF165-transfected vascular endothelial cells and islets in treatment of rats with diabetes
Ying Cheng, Yong-Feng Liu, Jia-Lin Zhang, Tie-Min Li, Ning Zhao
Ying Cheng, Yong-Feng Liu, Jia-Lin Zhang, Tie-Min Li, Ning Zhao,Organ Transplant Unit, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Supported bythe Doctoral Program Foundation of Ministry of Education, China, No. 20050159010
Correspondence to:Ying Cheng, Organ Transplant Unit, the First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang 110001, Liaoning Province, China. chengying75@sina.com
Received:2006-07-03 Accepted:2006-09-01
Abstract
AIM: To observed whether the elevated vascular endothelial growth factor (VEGF) expression, induced by VEGF165-transfected vascular endothelial cells (VEC), can affect the angiogene-sis and function of the grafts.
METHODS: The diabetic rats were divided into 3 groups, named group A, B and C, respectively. The rats in group A received 300-IEQ islets transplantation under the capsule of the kidney. The rats in group B underwent VEC and islets transplantation, while those in group C were transplanted with islets and VEC transfected by pIRES2-EGFP/VEGF165 plasmid. The levels of blood glucose and insulin were evaluated after operation every other day. Intravenous glucose tolerance test (IVGTT) was performed 10 d after transplantation. HE and immunohistochemical staining was used to detect the expression of insulin-6, VEGF and CD34 in the rat kidney ......
您现在查看是摘要介绍页，详见PDF附件(1210KB，7页)。